FGF-4 Antibody [Unconjugated]

Recombinant Human FGF‑4 (Catalog # 235-F4) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235). The ND50 is typically 0.3-0.9 µg/mL.

FGF‑4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human FGF‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF235) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FGF-4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FGF-4
  Ala31-Leu206
  Accession # P08620
• Specificity: Detects human FGF‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) FGF-6 is observed and less than 2% cross-reactivity with bovine FGF acidic, bovine FGF basic, rhFGF acidic, rhFGF basic, rh beta ‑ECGF, rhFGF-5, rhFGF‑7, rhFGF‑8b, recombinant mouse (rm) FGF‑8b, rmFGF‑8c, and rhFGF‑10 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF4
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.3-0.9 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-4 Antibody [Unconjugated]

• FGF4
• FGF-4
• fibroblast growth factor 4
• HBGF-4
• HBGF-4Transforming protein KS3
• heparin secretory transforming protein 1
• Heparin secretory-transforming protein 1
• Heparin-binding growth factor 4
• HST-1
• HST-1HSTF-1
• HSTF1fibroblast growth factor 4 splice isoform
• HSTFGF-4
• human stomach cancer, transforming factor from FGF-related oncogene
• kaposi sarcoma oncogene
• KFGF
• K-FGF
• KS3
• oncogene HST

Background

FGF-4, the product of a developmentally regulated gene (hst-1), is a member of the FGF family that is efficiently secreted. The gene for FGF-4 (also known as FGFK or K-FGF for Kaposi sarcoma-associated FGF) was initially discovered as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors (including stomach and colon cancers, hepatocellular carcinomas, and Kaposi’s sarcomas). FGF-4 does not seem to be expressed in normal adult tissues. However, expression of the gene is spatially and temporally regulated during embryonic development. The murine homologue of human FGF-4 has been cloned and shown to be 82% homologous to the human protein at the amino acid sequence level. Human FGF-4 has been shown to exhibit cross species activity.

In vitro, FGF-4 is mitogenic for fibroblasts and endothelial cells. FGF-4 has been shown to be a potent angiogenesis promoter in vivo. FGF-4 has potent transforming potential apparently through an autocrine mechanism of action. FGF-4 plays a key role in limb development and has been identified as the molecular mediator of the activities of the apical ectodermal ridge that is required for directing the outgrowth and patterning of vertebrate limbs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF-4 Antibody (AF235)(2)

Publications using AF235

• Mary J. Eaton, Eva Widerström-Noga, Stacey Quintero Wolfe Subarachnoid Transplant of the Human Neuronal hNT2.19 Serotonergic Cell Line Attenuates Behavioral Hypersensitivity without Affecting Motor Dysfunction after Severe Contusive Spinal Cord Injury Neurology Research International 2011-06-01 [PMID: 21799949]
• Benjamin L. Allen, Mark S. Filla, Alan C. Rapraeger Role of heparan sulfate as a tissue-specific regulator of FGF-4 and FGF receptor recognition The Journal of Cell Biology 2001-11-26 [PMID: 11724824]

Bioinformatics

• Gene Symbol: FGF4
• Uniprot:
  • P08620()