FGF-21 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

FGF-21 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human FGF-21 (R&D Systems, Catalog # 2539-FG)
  His29-Ser209
  Accession # Q9NSA1
• Specificity: Detects human FGF-21 in Western blots. In this format, approximately 20% cross‑reactivity with recombinant mouse (rm) FGF‑21 is observed and less than 2% cross-reactivity with rhFGF-3, -4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -18, -19, -20, -23, rmFGF-8b, rmFGF-15, rhFGF acidic, and rhFGF basic is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF21
• Purity: Antigen Affinity-purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Antigen Affinity-purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-21 Antibody [Biotin]

• FGF21
• FGF-21
• fibroblast growth factor 21

Background

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The c-DNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 show limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the C-terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).

1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
3. Huang, X. et al. (2006) Mol. Carcinog. 45:934.
4. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
5. GenBank Accession #: EAW52401 (2006).
6. Ford, A.M. et al. (2005) J. Clin. Invest. 115:1627.
7. Moore, D. D. (2007) Science 316:1436.
8. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
9. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
10. Lundasen, T. et al. (2007) Biochem. Biophys. Res. Commun. 360:437.
11. Badman, M.K. et al. (2007) Cell Metab. 5:426.
12. Inagaki, T. et al. (2007) Cell Metab. 5:415.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF21
• Uniprot:
  • Q9NSA1()