FGF-21 Antibody (OTI2F10)

Western Blot: FGF21 Antibody (2F10) [NBP2-00645] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF21 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF21.

Immunocytochemistry/Immunofluorescence: FGF-21 Antibody (2F10) [NBP2-00645] - Anti-FGF21 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGF21(RC204538) at 1:100

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] Staining of paraffin-embedded Human tonsil using anti-FGF21 mouse monoclonal antibody.

Flow Cytometry: FGF-21 Antibody (2F10) [NBP2-00645] - HEK293T cells transfected with either RC204538 overexpress plasmid (Red) or empty vector control plasmid(Blue) were immunostained by anti-FGF21 antibody and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGF21 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FGF21 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FGF21 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FGF21 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FGF21 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Human breast tissue using anti-FGF21 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: FGF21 Antibody (2F10) [NBP2-00645] - Staining of paraffin-embedded Human pancreas tissue using anti-FGF21 mouse monoclonal antibody.

Flow Cytometry: FGF21 Antibody (2F10) [NBP2-00645] - Analysis of Jurkat cells, using anti-FGF21 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI2F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.4 mg/ml

FGF-21 Antibody (OTI2F10) Summary

• Description: Novus Biologicals Mouse FGF-21 Antibody (OTI2F10) (NBP2-00645) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 29-209 of human FGF21(NP_061986) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGF21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.4 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FGF-21 Antibody (OTI2F10)

• FGF21
• FGF-21
• fibroblast growth factor 21

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF21
• Entrez:
  • 26291(Human)