PTP alpha/PTPRA Antibody

Immunohistochemistry: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of lung, non-small cell carcinoma,

Immunohistochemistry-Paraffin: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skeletal muscle after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, cortex, after heat-induced antigen retrieval.

Immunohistochemistry: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of breast carcinoma

Immunohistochemistry: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of skin, malignant melanoma,

• Reactivity: Hu, Pm
• Applications: IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PTP alpha/PTPRA Antibody Summary

• Immunogen: Synthetic 17 amino acid peptide from extracellular domain of human PTP alpha/PTPRA.
• Epitope: Extracellular Domain
• Specificity: Human PTP alpha/PTPRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 15 ug/ml.

Reactivity Notes

Expected reactivity based on percent sequence identity: Hamster, Canine, Bovine, Rabbit (88%), Mouse, Equine (82%)

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Product can be stored undiluted at 4C for up to 1 month.

Alternate Names for PTP alpha/PTPRA Antibody

• EC 3.1.3.48
• HEPTP
• HLPR
• LRP
• LRPHPTPalpha
• protein tyrosine phosphatase, receptor type, A
• Protein-tyrosine phosphatase alpha
• PTP alpha
• PTPAreceptor type, alpha polypeptide
• PTPase-alpha
• PTPLCA-related phosphatase
• PTPRA
• PTPRL2
• receptor-type tyrosine-protein phosphatase alpha
• RPTPA
• R-PTP-alpha
• tyrosine phosphatase alpha

Background

PTP alpha is encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRA