APLP-1 Antibody [Unconjugated]

APLP‑1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human APLP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using out protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of human brain (hippocampus) and mouse brain (hippocampus), loaded at 0.2 mg/mL. A specific band was detected for APLP‑1 at approximately 119 kDa (as indicated) using 25 µg/mL of Goat Anti-Human APLP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

APLP-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human APLP-1
  Gln34-Glu580
  Accession # P51693
• Specificity: Detects human APLP‑1 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse APLP-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APLP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APLP-1 Antibody [Unconjugated]

• amyloid beta (A4) precursor-like protein 1
• amyloid precursor-like protein 1
• amyloid-like protein 1
• APLP1
• APLP-1
• APLPAPLP-1

Background

APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and is 89% identical between human and mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APLP-1 Antibody (AF3129)(1)

Publication using AF3129

• L Schauenbur, F Liebsch, M Eravci, MC Mayer, C Weise, G Multhaup APLP1 is endoproteolytically cleaved by ?-secretase without previous ectodomain shedding Sci Rep, 2018;8(1):1916. 2018 [PMID: 29382944] (Western Blot, Human)

Bioinformatics

• Gene Symbol: APLP1
• Uniprot:
  • P51693()