Fc gamma RIII (CD16) Antibody

Fc gamma RIII (CD16) was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Fc gamma RIII (CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1960) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Fc gamma RIII (CD16) Antibody Summary

• Specificity: Detects Fc gamma RIIIA/B (CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Fc gamma RIIIB and rhCD32 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Fcgr3
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 µg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIII (CD16) Antibody

• CD16
• CD16A
• Fc fragment of IgG receptor IIIa
• Fc gamma RIII
• FCG3
• FCGR3
• FcgRIII
• FCR-10
• FCRIII
• FCRIIIA
• IGFR3
• IMD20

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10^‑8‑10^‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10^-6-10^-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
5. Ravetch, J. and L. Lanier (2000) Science 290:84.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RIII (CD16) Antibody (AF1960)(4)

Publications using AF1960

• Li, Y;Noto, D;Hoshino, Y;Mizuno, M;Yoshikawa, S;Miyake, S; Immunoglobulin directly enhances differentiation of oligodendrocyte-precursor cells and remyelination Scientific reports 2023-06-09 [PMID: 37296298] (Flow Cytometry, Mouse)
• Laura Lopez‐Sanz, Susana Bernal, Luna Jimenez‐Castilla, Ignacio Prieto, Sara La Manna, Sergio Gomez‐Lopez et al. Fc gamma receptor activation mediates vascular inflammation and abdominal aortic aneurysm development Clinical and Translational Medicine 2021-07-04 [PMID: 34323424]
• Menna R. Clatworthy, Caren E. Petrie Aronin, Rebeccah J. Mathews, Nicole Y Morgan, Kenneth G.C. Smith, Ronald N. Germain Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes Nature Medicine 2014-12-01 [PMID: 25384086]
• Ichii O, Konno A, Sasaki N, Endoh D, Hashimoto Y, Kon Y Altered balance of inhibitory and active Fc gamma receptors in murine autoimmune glomerulonephritis. Kidney Int., 2008-05-07;74(3):339-47. 2008-05-07 [PMID: 18463609] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: Fcgr3
• Uniprot:
  • P08508()