Fc gamma RIIA/CD32a Antibody (8.7) [Biotin]

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 8.7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

Fc gamma RIIA/CD32a Antibody (8.7) [Biotin] Summary

• Immunogen: K562 and FcgRII+L cells
• Localization: Cell Surface
• Specificity: This MAb reacts with a CD32 (FcgRII) epitope distinct from that defined by MAb 8.26 and the epitope overlaps with that of MAb 7.30 (cluster 4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (Fc?RII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR2A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Fc gamma RIIA/CD32a Antibody (8.7) [Biotin]

• CD32 antigen
• CD32a
• CD32MGC23887
• CDw32fc-gamma-RIIa
• Fc fragment of IgG, low affinity IIa, receptor (CD32)
• Fc fragment of IgG, low affinity IIa, receptor for (CD32)
• Fc gamma RIIA
• FCG2
• Fc-gamma RII-a
• Fc-gamma-RIIa
• FcGR
• FCGR2
• FCGR2A
• FCGR2A1
• FcgRIIA
• FCRIIA
• fcRII-a
• IGFR2MGC30032
• IgG Fc receptor II-a
• Immunoglobulin G Fc receptor II
• low affinity immunoglobulin gamma Fc region receptor II-a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Fc gamma RIIA/CD32a Antibody (NBP2-47831B). (Showing 1 - 1 of 1 FAQ).

1. Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
   • For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

Bioinformatics

• Gene Symbol: FCGR2A