Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

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1 μg/lane of Recombinant Human Fc gamma RIIIA/CD16a was resolved with SDS‑PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 40‑50 kDa.
Recombinant Human Fc gamma RIIIA/CD16a (Catalog # 4325-FC) binds human IgG with an estimated Kd <50 nM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Fc gamma RIIIA/CD16a Protein, CF Summary

Additional Information
Isoform V176
Details of Functionality
Measured by its ability to bind human IgG with an estimated Kd <50 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RIIIA/CD16a protein
Gly17-Gln208, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Gly17
Protein/Peptide Type
Recombinant Proteins
Gene
FCGR3A
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-50 kDa, reducing conditions
Publications
Read Publications using
4325-FC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

  • CD16a antigen
  • CD16a
  • CD16FCRIIIA
  • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
  • Fc gamma receptor III-A
  • Fc gamma RIIIA
  • FCG3
  • Fc-gamma receptor III-2 (CD 16)
  • Fc-gamma receptor IIIb (CD16)
  • Fc-gamma RIII
  • Fc-gamma RIIIa
  • Fc-gamma RIII-alpha
  • FCGR3
  • FCGR3A
  • FCGRIII
  • FcgRIIIA
  • FcR-10
  • FCRIII
  • FcRIIIa
  • IGFR3FcgammaRIIIA
  • immunoglobulin G Fc receptor III
  • low affinity III, receptor for (CD16)
  • low affinity immunoglobulin gamma Fc region receptor III-A
  • neutrophil-specific antigen NA

Background

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 191 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 22 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (176V) and low binding (176F) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # 4325-FC is expressed as the 176V isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).

  1. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19. 
  2. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481. 
  3. Wu, J. et al. (1997) J. Clin. Invest. 100:1059. 
  4. Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664. 
  5. Kim, M.-K. et al. (2003) Blood 101:4479. 
  6. Lanier, L.L. et al. (1989) Nature 342:803.
  7. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
  8. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
  9. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
  10. Masuda, M. et al. (2006) Atherosclerosis 188:377.
  11. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.

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Publications for Fc gamma RIIIA/CD16a (4325-FC)(13)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 6 applications: Binding Assay, Bioassay, ELISA (Capture), EMSA, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.


Filter By Application
Binding Assay
(1)
Bioassay
(7)
ELISA (Capture)
(1)
EMSA
(1)
Surface Plasmon Resonance
(2)
Surface Plasmon Resonance (SPR
(1)
All Applications
Filter By Species
Human
(10)
N/A
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 4325-FC Applications Species
PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus J. Immunol., 2019;0(0):. 2019 [PMID: 30683699] (ELISA (Capture), Human) ELISA (Capture) Human
G Wozniak-Kn, G Stadlmayr, JW Perthold, K Stadlbauer, M Gotsmy, S Becker, F Rüker An antibody with Fab-constant domains exchanged for a pair of CH3 domains PLoS ONE, 2018;13(4):e0195442. 2018 [PMID: 29630643] (Bioassay) Bioassay
F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus,,,, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies Cancer Cell, 2018;0(0):. 2018 [PMID: 29576375] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
C Lee, M Jeong, JJ Lee, S Seo, SC Cho, W Zhang, O Jaquez Glycosylation profile and biological activity of Remicade� compared with Flixabi� and Remsima� MAbs, 2017;0(0):0. 2017 [PMID: 28640663] (Binding Assay, N/A) Binding Assay N/A
C Le Clorenn, H Bazin, O Dubreuil, C Larbouret, C Ogier, Y Lazrek, V Garambois, MA Poul, P Mondon, JM Barret, G Mathis, JF Prost, A Pèlegrin, T Chardès Neuregulin 1 allosterically enhances the anti-tumor effects of the non-competing anti-HER3 antibody 9F7-F11 by increasing its binding to HER3 Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28507002] (Bioassay, Human) Bioassay Human
Lohse S, Meyer S, Meulenbroek L, Jansen J, Nederend M, Kretschmer A, Klausz K, Moginger U, Derer S, Rosner T, Kellner C, Schewe D, Sondermann P, Tiwari S, Kolarich D, Peipp M, Leusen J, Valerius T An Anti-EGFR IgA That Displays Improved Pharmacokinetics and Myeloid Effector Cell Engagement In Vivo. Cancer Res, 2016;76(2):403-17. 2016 [PMID: 26634925] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Yang Z, Ramsey J, Hamza T, Zhang Y, Li S, Yfantis H, Lee D, Hernandez L, Seghezzi W, Furneisen J, Davis N, Therien A, Feng H Mechanisms of protection against Clostridium difficile infection by the monoclonal antitoxin antibodies actoxumab and bezlotoxumab. Infect Immun, 2015;83(2):822-31. 2015 [PMID: 25486992] (Bioassay, Human) Bioassay Human
Anikeeva N, Steblyanko M, Fayngerts S, Kopylova N, Marshall D, Powers G, Sato T, Campbell K, Sykulev Y Integrin receptors on tumor cells facilitate NK cell-mediated antibody-dependent cytotoxicity. Eur J Immunol, 2014;44(8):2331-9. 2014 [PMID: 24810893] (Bioassay, Human) Bioassay Human
Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases. J Immunol, 2014;192(9):4083-92. 2014 [PMID: 24670803] (Bioassay, Human) Bioassay Human
Lajoie L, Congy-Jolivet N, Bolzec A, Gouilleux-Gruart V, Sicard E, Sung H, Peiretti F, Moreau T, Vie H, Clemenceau B, Thibault G ADAM17-mediated shedding of FcgammaRIIIA on human NK cells: identification of the cleavage site and relationship with activation. J Immunol, 2014;192(2):741-51. 2014 [PMID: 24337742] (Bioassay, Human) Bioassay Human
Show All 13 Publications.

Reviews for Fc gamma RIIIA/CD16a (4325-FC) (0)

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FAQs for Fc gamma RIIIA/CD16a (4325-FC). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (http://www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.
    • The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.

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Bioinformatics

Gene Symbol FCGR3A
Uniprot