NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Fc gamma RII/CD32 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Fc gamma RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1330) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1330-SP | |||
F0107). View our protocol for Staining Membrane-associated Proteins. " alt="Whole blood granulocytes were stained with Goat Anti-Human Fc gamma RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1875, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1875-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1330-CD-050/CF | |||
| Carrier Free | 1330-CD-01M/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70709 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20159-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84589-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89456-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47830-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47831-0.1mg | |||
![Western Blot: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Western-Blot-NBP1-47735-img0022.jpg "Western Blot: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.")
![Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunocytochemistry-Immunofluorescence-NBP1-47735-img0013.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human prostate tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0021.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0014.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human breast tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0015.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human colon tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0016.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human endometrium tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0017.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human Kidney tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0018.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human lung tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0019.jpg)
![Immunohistochemistry-Paraffin: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Staining of paraffin-embedded Human pancreas tissue using anti-FCGR2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunohistochemistry-Paraffin-NBP1-47735-img0020.jpg)
![Immunoprecipitation: Fc gamma RIIA/CD32a Antibody (9G5) [NBP1-47735] - Lane 1: Negative Control Lane 2: NBP1-47738 Lane 3: NBP1-47739 Lane 4: NBP1-47740 Lane 5: NBP1-47741 Lane 6: NBP1-47742 Lane 7: NBP1-47734 Lane 8: NBP1-47735 Lane 9: NBP1-47736 Lane 10: NBP1-47737 Lane 11: NBP1-47743 Lane 12: NBP1-47744](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-9G5-Immunoprecipitation-NBP1-47735-img0023.jpg)
![Flow Cytometry: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] - Paraformaldehyde fixed U937 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of IV.3 (NBP3-20159, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20159_rabbit-fc-gamma-riia-cd32a-mab-iv-3-chimeric-azide-bsa-free-2742023924258.png "Flow Cytometry: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] - Paraformaldehyde fixed U937 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of IV.3 (NBP3-20159, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (IV.3) - Chimeric - Azide and BSA Free [NBP3-20159] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of IV.3 (NBP3-20159) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20159, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20159_rabbit-fc-gamma-riia-cd32a-mab-iv-3-chimeric-azide-bsa-free-2742023924281.png)
![Western Blot: Fc gamma RIIA/CD32a Antibody (020) [NBP2-89456] - Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Fc-gamma-RIIA-CD32a-Antibody-020-Western-Blot-NBP2-89456-img0001.jpg "Western Blot: Fc gamma RIIA/CD32a Antibody (020) [NBP2-89456] - Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (JE64-17) [NBP3-33141] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Fc gamma RIIA/CD32a antibody (NBP3-33141) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33141) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33141_rabbit-fc-gamma-riia-cd32a-mab-je64-17-29820241426266.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (JE64-17) [NBP3-33141] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Fc gamma RIIA/CD32a antibody (NBP3-33141) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33141) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
