Recombinant Porcine Erythropoietin/EPO Protein

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, ELISA
Concentration
LYOPH

Order Details

Recombinant Porcine Erythropoietin/EPO Protein Summary

Description
Recombinant pig erythropoietin expressed in Pichia pastoris and purified by ion-exchange chromatography.

Source:Pichia pastoris

Amino Acid Sequence:18.6 kDa recombinant protein corresponding to aa 27 - 194 of native porcine erythropoietin (EPO).

Preparation
Method
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Source
Pichia pastoris
Protein/Peptide Type
Recombinant Protein
Gene
EPO
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot
Theoretical MW
18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
PBS
Preservative
10% Trehalose
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.

Alternate Names for Recombinant Porcine Erythropoietin/EPO Protein

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (Epo) stimulates red blood cell production by docking with its cognate receptor on the erythroid progenitor cell and triggering an array of signaling pathways that inhibit apoptosis and promote cell proliferation and differentiation. In its pharmaceutical forms, epoetin and darbepoetin, Epo is widely used to treat various anemias, including those associated with cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EPO