287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.2 units/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human Erythropoietin/EPO Polyclonal Antibody (Catalog # AB-286-NA). The ND₅₀ is typically <3 µg/mL." class="big_lightbox"> 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.2 units/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human Erythropoietin/EPO Polyclonal Antibody (Catalog # AB-286-NA). The ND₅₀ is typically <3 µg/mL." alt="Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.2 units/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human Erythropoietin/EPO Polyclonal Antibody (Catalog # AB-286-NA). The ND₅₀ is typically <3 µg/mL." class="big_thumb" />

VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_lightbox"> 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND₅₀ is typically 0.5-1.5 µg/mL." alt="Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_thumb" />

959-ME) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB959). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox"> 959-ME) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB959). The ND₅₀ is typically 0.2-0.8 µg/mL." alt="Recombinant Mouse Erythropoietin/EPO (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB959). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />

959-ME) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_lightbox"> 959-ME) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591). The ND₅₀ is typically 0.15-0.75 µg/mL." alt="Recombinant Mouse Erythropoietin/EPO (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_thumb" />

959-ME) was serially diluted and captured by Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Erythropoietin/EPO Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 959-ME) was serially diluted and captured by Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Erythropoietin/EPO Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Erythropoietin/EPO was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB2873) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

287-TC) stimulates proliferation in theTF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB2872). The ND₅₀ is typically 0.02-0.2 µg/mL." class="big_lightbox"> 287-TC) stimulates proliferation in theTF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB2872). The ND₅₀ is typically 0.02-0.2 µg/mL." alt="Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in theTF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB2872). The ND₅₀ is typically 0.02-0.2 µg/mL." class="big_thumb" />