ERR gamma/NR3B3 Antibody - BSA Free

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERR gamma antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853] - Rat spleen lysate/extract.

IHC-Wm- ERR gamma/NR3B3 Antibody [NBP1-32853] - 2 days-post-fertilization zebrafish embryo.ERR gamma antibody dilution: 1:200.

IHC-Wm-ERR gamma/NR3B3 Antibody [NBP1-32853] -2 days-post-fertilization zebrafish embryo.ERR gamma antibody dilution: 1:200.

• Reactivity: Hu, Mu, Rt, Ze, Ch
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ERR gamma/NR3B3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ERR gamma/NR3B3 Antibody - BSA Free (NBP1-32853) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ERR gamma/NR3B3. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESRRG
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Whole-Mount Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ERR gamma/NR3B3 Antibody - BSA Free

• DKFZp781L1617
• ERR gamma
• ERR gamma-2
• ERR3
• ERRG2
• ERRgamma
• ESRRG
• Estrogen receptor-related protein 3
• estrogen-related receptor gamma
• FLJ16023
• KIAA0832
• NR3B3
• Nuclear receptor subfamily 3 group B member 3

Background

Estrogen related receptor (ERR gamma), a NR3 Steroid Receptor is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members (1,2, and 3) share an almost identical DNA binding domain, which has 68% amino acid identity with that of estrogen receptor. Studies involving the crystal structure of the LBD have shown a complex between ERR3 and SRC1. ERR gamma has been suggested to affect differentiation of the brain, heart, and kidney. ERR gamma binds as a monomer to an extended half site of the ERRE type (TCAAGGTCA). ERR gamma has been shown to interact with PGC1 alpha and has been implicated in the regulation of mitochondrial energy metabolism. In humans, ERR gamma pre mRNA undergoes extensive alternative splicing at the 5 prime end, yielding at least six mRNA splice variants and two protein isoforms that differ by 23 amino acids in the N terminus. ERR gamma has been shown to be over expressed in breast tumors, and its expression is correlated with levels of ErbB4, a likely indicator of preferred clinical course. As a result, ERR gamma has been suggested to be a potential biomarker for favorable clinical course and, possibly, hormonal sensitivity, and as a candidate target for therapeutic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for ERR gamma/NR3B3 Antibody (NBP1-32853). (Showing 1 - 1 of 1 FAQ).

1. I am looking for an antibody to detect human ERRgamma by Western blot in primary human muscle cells. There are several antibodies indicating for WB from Novus. Could you recommend the best one for me to order. thanks!
   • NBP1-32853 works well for Western blot in human tissue. The positive control I would recommend is Hela, A431, HepG2 or Raji cells.

Bioinformatics

• Gene Symbol: ESRRG