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ERO1L Antibody (V23P3C9*H2) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Eq, ShSpecies Glossary
Applications WB, ELISA, IHC
Clone
V23P3C9*H2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50388AF350
Alexa Fluor 405
NBP2-50388AF405
Alexa Fluor 488
NBP2-50388AF488
Alexa Fluor 532
NBP2-50388AF532
Alexa Fluor 594
NBP2-50388AF594
Alexa Fluor 647
NBP2-50388AF647
Alexa Fluor 700
NBP2-50388AF700
Alexa Fluor 750
NBP2-50388AF750
Biotin
NBP2-50388B
DyLight 350
NBP2-50388UV
DyLight 488
NBP2-50388G
DyLight 550
NBP2-50388R
DyLight 594
NBP2-50388DL594
DyLight 650
NBP2-50388C
DyLight 680
NBP2-50388FR
DyLight 755
NBP2-50388IR
FITC
NBP2-50388F
HRP
NBP2-50388H
Janelia Fluor 549
NBP2-50388JF549
Janelia Fluor 646
NBP2-50388JF646
Unconjugated
NBP2-50388
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ERO1L Antibody (V23P3C9*H2) [DyLight 405] Summary

Immunogen
Peptide Sequence - KLIANMPESG (peptide immunogen is identical in rat (Rattus norvegicus), mouse (Mus musculus), sheep (Ovis aries), cow (Bos taurus), dog (Canis familiaris), pig (Sus scrofa) and horse (Equus caballus)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ERO1A
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

The clone number is listed as V23P3C9*H2 in the scientific literature.

Alternate Names for ERO1L Antibody (V23P3C9*H2) [DyLight 405]

  • EC 1.8.4
  • EC 1.8.4.-
  • Endoplasmic oxidoreductin-1-like protein
  • ERO1 (S. cerevisiae)-like
  • ERO1A
  • ERO1-alpha
  • ERO1L alpha
  • ERO1-l alpha
  • ERO1L
  • ERO1-L
  • ERO1-L-alpha
  • ERO1-like (S. cerevisiae)
  • ERO1-like alpha
  • ERO1-like protein alpha
  • ERO1-like
  • oxidoreductin-1-L-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: PDI Antibody (RL90) [NB300-517] - Staining of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes.
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Immunohistochemistry-Paraffin: PADI1 Antibody [NBP2-37761] - Staining of human colon shows low expression as expected.
Immunohistochemistry-Paraffin: PADI1 Antibody [NBP2-37761] - Staining of human esophagus shows cytoplasmic and nuclear positivity in squamous epithelial cells.
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Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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Western Blot: ERP44 Antibody (3C7) [H00023071-M01] - TXNDC4 monoclonal antibody (M01), clone 3C7 Analysis of TXNDC4 expression in K-562.
Immunohistochemistry-Paraffin: ERP44 Antibody (3C7) [H00023071-M01] - Analysis of monoclonal antibody to TXNDC4 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: ERP44 Antibody (3C7) [H00023071-M01] - TXNDC4 monoclonal antibody (M01), clone 3C7. Analysis of TXNDC4 expression in A-431.
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NBP2-50389
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Immunohistochemistry-Paraffin: ERO1LB Antibody (M37-P5D11) [NBP2-50389] - Immunohistochemistry images are showing a stronger cytoplasmic staining of ERO1LB in (A) primary colorectal cancer compared to (B) normal colon mucosa using ERO1LB Antibody (M37-P5D11). IHC was performed on formalin-fixed, paraffin-embedded tissue sections. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).
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NBP1-84796
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NBP2-02117
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p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
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IP3R1 Antibody
NBP1-21398
Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.
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ERO1L Antibody
(V23P3C9*H2) [DyLight ...
NBP2-50388V
ERO1L Antibody (V23P3C9*H2) [DyLight 405]
Species: Hu, Mu, Rt, Po, Ca, Eq, Sh
Applications: WB, ELISA, IHC

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50388AF350
Alexa Fluor 405 NBP2-50388AF405
Alexa Fluor 488 NBP2-50388AF488
Alexa Fluor 532 NBP2-50388AF532
Alexa Fluor 594 NBP2-50388AF594
Alexa Fluor 647 NBP2-50388AF647
Alexa Fluor 700 NBP2-50388AF700
Alexa Fluor 750 NBP2-50388AF750
Biotin NBP2-50388B
DyLight 350 NBP2-50388UV
DyLight 405 NBP2-50388V
DyLight 488 NBP2-50388G
DyLight 550 NBP2-50388R
DyLight 594 NBP2-50388DL594
DyLight 650 NBP2-50388C
DyLight 680 NBP2-50388FR
DyLight 755 NBP2-50388IR
FITC NBP2-50388F
HRP NBP2-50388H
Janelia Fluor 549 NBP2-50388JF549
Janelia Fluor 646 NBP2-50388JF646
Learn about Custom Antibody Labeling

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ERO1 Activity: A Potential Source of ER-Derived Oxidative Stress.
Disulfide bond formation is a pivotal step in the maturation and release of secretory proteins that are controlled by specific endoplasmic reticulum (ER) resident enzymes. An important element in this process is ERO (ER oxidoreduction), a glycosylated...  Read full blog post.
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Bioinformatics

Gene Symbol ERO1A