ERO1L Antibody (V23P3C9*H2) [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt, Po, Ca, Eq, Sh
• Applications: WB, ELISA, IHC
• Clone: V23P3C9*H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

ERO1L Antibody (V23P3C9*H2) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Peptide Sequence - KLIANMPESG (peptide immunogen is identical in rat (Rattus norvegicus), mouse (Mus musculus), sheep (Ovis aries), bovine (Bos taurus), canine (Canis familiaris), porcine (Sus scrofa) and equine (Equus caballus)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERO1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for ERO1L Antibody (V23P3C9*H2) [CoraFluor™ 1]

• EC 1.8.4
• EC 1.8.4.-
• Endoplasmic oxidoreductin-1-like protein
• ERO1 (S. cerevisiae)-like
• ERO1A
• ERO1-alpha
• ERO1L alpha
• ERO1-l alpha
• ERO1L
• ERO1-L
• ERO1-L-alpha
• ERO1-like (S. cerevisiae)
• ERO1-like alpha
• ERO1-like protein alpha
• ERO1-like
• oxidoreductin-1-L-alpha

Background

Perhaps the most distinctive feature of protein folding in the ER is the abundance of disulfide bonds that must form during maturation of proteins traveling along the secretory pathway. Formation of disulfide bonds is a redox reaction. Thus, to match the flux of disulfide bonds that exit from the ER by virtue of protein secretion, a flux of oxidizing equivalents into the ER is required. In eukaryotic cells, the essential protein relay supporting this flux, and hence disulfide bond formation, involves endoplasmic reticulum oxidoreductin 1 (Ero1) and protein disulfide isomerase (PDI). The temporal pattern of hypoxic ERO1-L alpha induction is very similar to that of genes triggered by the hypoxia inducible transcription factor (HIF-1) and is characteristically mimicked by cobalt and by deferoxamine, but is absent in cells with a defective aryl hydrocarbon receptor translocator (ARNT, HIF-1 alpha). We speculate from these findings that the expression of ERO1-L alpha is probably regulated via the HIF-pathway and thus belongs to the family of classic oxygen regulated genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERO1A