ERO1L Antibody (V23P3C9*H2) [DyLight 755]

• Reactivity: Hu, Mu, Rt, Po, Ca, Eq, Sh
• Applications: WB, ELISA, IHC
• Clone: V23P3C9*H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 755

ERO1L Antibody (V23P3C9*H2) [DyLight 755] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Peptide Sequence - KLIANMPESG (peptide immunogen is identical in rat (Rattus norvegicus), mouse (Mus musculus), sheep (Ovis aries), bovine (Bos taurus), canine (Canis familiaris), porcine (Sus scrofa) and equine (Equus caballus)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERO1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-50388. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for ERO1L Antibody (V23P3C9*H2) [DyLight 755]

• EC 1.8.4
• EC 1.8.4.-
• Endoplasmic oxidoreductin-1-like protein
• ERO1 (S. cerevisiae)-like
• ERO1A
• ERO1-alpha
• ERO1L alpha
• ERO1-l alpha
• ERO1L
• ERO1-L
• ERO1-L-alpha
• ERO1-like (S. cerevisiae)
• ERO1-like alpha
• ERO1-like protein alpha
• ERO1-like
• oxidoreductin-1-L-alpha

Background

Perhaps the most distinctive feature of protein folding in the ER is the abundance of disulfide bonds that must form during maturation of proteins traveling along the secretory pathway. Formation of disulfide bonds is a redox reaction. Thus, to match the flux of disulfide bonds that exit from the ER by virtue of protein secretion, a flux of oxidizing equivalents into the ER is required. In eukaryotic cells, the essential protein relay supporting this flux, and hence disulfide bond formation, involves endoplasmic reticulum oxidoreductin 1 (Ero1) and protein disulfide isomerase (PDI). The temporal pattern of hypoxic ERO1-L alpha induction is very similar to that of genes triggered by the hypoxia inducible transcription factor (HIF-1) and is characteristically mimicked by cobalt and by deferoxamine, but is absent in cells with a defective aryl hydrocarbon receptor translocator (ARNT, HIF-1 alpha). We speculate from these findings that the expression of ERO1-L alpha is probably regulated via the HIF-pathway and thus belongs to the family of classic oxygen regulated genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERO1A