ER alpha/NR3A1 Antibody

Images

 
Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Analysis of extracts from MCF7 cells using Estrogen Receptor-alpha (Ab-106) antibody and Estrogen Receptor-alpha (phospho-Ser106) antibody.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Analysis of paraffin- embedded human breast carcinoma tissue using Estrogen Receptor-alpha antibody.
Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Analysis of extracts from MCF-7, SKOV3, and Rat Hippocampus.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

ER alpha/NR3A1 Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor-alpha around aa.106 (S-P- S-P-L).
Specificity
Estrogen Receptor alpha (specific to a.a. 106) detects endogenous levels of total Estrogen Receptor alpha protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ESR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5001:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ER alpha/NR3A1 Antibody

  • ER alpha
  • ER
  • Era
  • ER-alpha
  • ESR1
  • ESRESRA
  • Estradiol receptor
  • estrogen receptor 1
  • estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
  • estrogen receptor alpha delta 4 +49 isoform
  • estrogen receptor alpha delta 4*5,6,7*/654 isoform
  • estrogen receptor alpha
  • estrogen receptor
  • NR3A1
  • NR3A1DKFZp686N23123
  • Nuclear receptor subfamily 3 group A member 1

Background

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ESR1
Entrez
Uniprot