ER alpha/NR3A1 Antibody (S21-V)

Immunohistochemistry-Paraffin: Estrogen Receptor Antibody (S21-V) [NBP1-30153] - human of breast tumor tissue stained with Estrogen Receptor antibody shows strong positive nuclear immunostaining.

• Reactivity: Hu
• Applications: IHC
• Clone: S21-V
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ER alpha/NR3A1 Antibody (S21-V) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Immunogen: Peptide derived from C-terminal sequence of human ER alpha/NR3A1. Antibody recognizes the epitope between Gln580 - Thr594.
• Epitope: Gln580 - Thr594
• Specificity: This antibody is specific for the C terminus of Estrogen Receptor.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ESR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-200

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for ER alpha/NR3A1 Antibody (S21-V)

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1
• Entrez:
  • 2099(Human)
• Uniprot:
  • P03372()