ER alpha/NR3A1 Antibody (6A6E8)

Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of esophageal cancer tissues using ESR1 mouse mAb with DAB staining.

Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)

Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of breast cancer tissues using ESR1 mouse mAb with DAB staining.

ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clone: 6A6E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ER alpha/NR3A1 Antibody (6A6E8) Summary

• Immunogen: Purified recombinant fragment of human ESR1 (AA: 2-185) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:200
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1-1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
• Theoretical MW: 66.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for ER alpha/NR3A1 Antibody (6A6E8)

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1