| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow |
| Clone | 001 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human EphB4 (NP_004435.3; Met1-Ala539). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | EPHB4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-89352AF350 | |
| Alexa Fluor 405 | NBP2-89352AF405 | |
| Alexa Fluor 488 | NBP2-89352AF488 | |
| Alexa Fluor 532 | NBP2-89352AF532 | |
| Alexa Fluor 594 | NBP2-89352AF594 | |
| Alexa Fluor 647 | NBP2-89352AF647 | |
| Alexa Fluor 700 | NBP2-89352AF700 | |
| Alexa Fluor 750 | NBP2-89352AF750 | |
| Allophycocyanin | NBP2-89352APC | |
| Biotin | NBP2-89352B | |
| DyLight 350 | NBP2-89352UV | |
| DyLight 405 | NBP2-89352V | |
| DyLight 488 | NBP2-89352G | |
| DyLight 550 | NBP2-89352R | |
| DyLight 594 | NBP2-89352DL594 | |
| DyLight 650 | NBP2-89352C | |
| DyLight 680 | NBP2-89352FR | |
| DyLight 755 | NBP2-89352IR | |
| FITC | NBP2-89352F | |
| HRP | NBP2-89352H | |
| Janelia Fluor 549 | NBP2-89352JF549 | |
| Janelia Fluor 646 | NBP2-89352JF646 | |
| PE | NBP2-89352PE | |
| PerCP | NBP2-89352PCP |
Diseases for EphB4 Antibody (NBP2-89352B)Discover more about diseases related to EphB4 Antibody (NBP2-89352B).
| Pathways for EphB4 Antibody (NBP2-89352B)View related products by pathway.
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PTMs for EphB4 Antibody (NBP2-89352B)Learn more about PTMs related to EphB4 Antibody (NBP2-89352B).
| Research Areas for EphB4 Antibody (NBP2-89352B)Find related products by research area.
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Vimentin: Regulating EMT and Cancer Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea... Read full blog post. |
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| Gene Symbol | EPHB4 |
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Western Blot: EphA2 Antibody [NBP2-24489] - Analysis of human EphA2 in human ovary lysate in the absence of immunizing peptide using NBP2-24489 at 1 ug/ml.](http://images.novusbio.com/images2/EphA2-Antibody-Western-Blot-NBP2-24489-img0004.jpg)
![Immunohistochemistry-Paraffin: EphA2 Antibody [NBP2-24489] - Analysis of human pancreas using NBP2-24489 at 10 ug/ml.](http://images.novusbio.com/images2/EphA2-Antibody-Immunohistochemistry-Paraffin-NBP2-24489-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Immunohistochemistry-Paraffin-NBP1-81838-img0010.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in control (vector only transfected HEK293T lysate) and QPCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0011.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0006.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Western-Blot-NBP1-47842-img0011.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Endometrium tissue within the normal limits using antiMAPK1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,Dilution 1:50)](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Immunohistochemistry-Paraffin-NBP1-47842-img0019.jpg)
![Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK2.](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Western-Blot-NBP1-47842-img0010.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.](http://images.novusbio.com/images2/KRAS-Antibody-(3B10-2F2)-Western-Blot-H00003845-M01-img0004.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/KRAS Antibody (3B10-2F2)-Western Blot-H00003845-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of monoclonal antibody to KRAS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/KRAS-Antibody-(3B10-2F2)-Immunocytochemistry-Immunofluorescence-H00003845-M01-img0006.jpg)