ENGASE Antibody

Immunocytochemistry/Immunofluorescence: ENGASE Antibody [NBP1-93948] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.

Immunohistochemistry-Paraffin: ENGASE Antibody [NBP1-93948] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ENGASE Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DKDTTKPISFYLSSLEELLAWKPRLEDGFNVALEPLACRQPPLSSQRPRTLLCHDMMGGYLDDRFIQGSVVQTPYAFYHWQCIDVFVYFSHHTVTIPPVGWTNTAHR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENGASE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ENGASE Protein (NBP1-93948PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ENGASE Antibody

• cytosolic endo-beta-N-acetylglucosaminidase
• endo-beta-N-acetylglucosaminidase
• FLJ21865

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENGASE