TRAPPC9 Antibody

• Reactivity: Hu, Mu, Bv, Ca, Ch, Pm, Xp
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRAPPC9 Antibody Summary

• Immunogen: Amino acids 282-299 (KKDFVGLDTDSRHYKKRC) of human NIBP was used as the immunogen.
• Predicted Species: Xenopus (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAPPC9
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot reported in literature (See Zahoor et al, 2010 for details). Confocal immunofluorescence: Cells were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.5 % Triton X-100 for 20 min at room temperature (RT). The cells were blocked with PBS containing 10 % BSA and probed with NIBP pAB for 1 hour at RT. After washing with PBS, cellswere incubated with Alexa conjugated goat anti-mouse antibodies.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in human (isoform CRA_A and CRA_b), chimpanzee, monkey, mouse, opossum, rat and dog; xenopus (94%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole Rabbit antisera with 50% Glycerol
• Preservative: 0.025% Sodium Azide
• Purity: Unpurified

Notes

1.The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_A and CRA_b), chimpanzee, monkey, mouse, opossum, rat and dog and 94% homologous in xenopus. 2. Confocal immunofluorescence: Cells were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.5 % Triton X-100 for 20 min at room temperature (RT). The cells were blocked with PBS containing 10 % BSA and probed with NIBP pAB for 1 hour at RT. After washing with PBS, cellswere incubated with Alexa conjugated goat anti-mouse antibodies. See Zahoor et al for details (2010).

Alternate Names for TRAPPC9 Antibody

• IKBKBBP
• IKK2 binding protein
• KIAA1882IBP
• MRT13MGC4769
• NIBPMGC4737
• NIK- and IKBKB-binding protein
• NIK and IKK-beta binding protein
• T1
• trafficking protein particle complex 9
• trafficking protein particle complex subunit 9
• TRAPP 120 kDa subunit
• TRS120
• Tularik gene 1 protein

Background

NIBP is a 1246 amino acid intracellular regulatory protein. Reports suggest that NIBP physically interacts with NIK, IKK (beta), but not IKK (alpha) or IKK (gamma) and thus acts as a novel enhancer of cytokine-induced NF-(kappa) B signaling pathway. Due to its neuronal expression NIBP is considered as a potential target for modulating the NF-(kappa) B signaling cascade in neuronal pathologies dependent upon abnormal activation of this pathway. NIBP is mainly expressed in brain, muscle, heart, and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAPPC9 Antibody (NB100-55735)(1)

Publication using NB100-55735

• Zahoor MA, Yamane D, Mohamed YM et al. Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKbeta-binding protein. J Gen Virol. 2010-08-01 [PMID: 20444997] (WB)
  
  Details:
  IF (Fig 4): NIBP-FLAG transiently transfected LB9.K (bovine epithelial) cell line. Note: NIBP pAB was transfected validated in LB9.K cells by immunofluorescence. IP & IP/WB (Fig 2): Endogenous NIBP in LB9.K cells infected with bovine viruses was immunopre

Bioinformatics

• Gene Symbol: TRAPPC9
• Entrez:
  • 83696(Human)
• Uniprot:
  • Q96Q05(Human)