TRAPPC9 Antibody

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Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, Ch, Pm, XpSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TRAPPC9 Antibody Summary

Immunogen
Amino acids 282-299 (KKDFVGLDTDSRHYKKRC) of human NIBP was used as the immunogen.
Predicted Species
Xenopus (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRAPPC9
Purity
Unpurified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot reported in literature (See Zahoor et al, 2010 for details). Confocal immunofluorescence: Cells were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.5 % Triton X-100 for 20 min at room temperature (RT). The cells were blocked with PBS containing 10 % BSA and probed with NIBP pAB for 1 hour at RT. After washing with PBS, cellswere incubated with Alexa conjugated goat anti-mouse antibodies.
Publications
Read Publication using NB100-55735.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_A and CRA_b), chimpanzee, monkey, mouse, opossum, rat and dog and 94% homologous in xenopus.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole Rabbit antisera with 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified

Notes

1.The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_A and CRA_b), chimpanzee, monkey, mouse, opossum, rat and dog and 94% homologous in xenopus. 2. Confocal immunofluorescence: Cells were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.5 % Triton X-100 for 20 min at room temperature (RT). The cells were blocked with PBS containing 10 % BSA and probed with NIBP pAB for 1 hour at RT. After washing with PBS, cellswere incubated with Alexa conjugated goat anti-mouse antibodies. See Zahoor et al for details (2010).

Alternate Names for TRAPPC9 Antibody

  • IKBKBBP
  • IKK2 binding protein
  • KIAA1882IBP
  • MRT13MGC4769
  • NIBPMGC4737
  • NIK- and IKBKB-binding protein
  • NIK and IKK-beta binding protein
  • T1
  • trafficking protein particle complex 9
  • trafficking protein particle complex subunit 9
  • TRAPP 120 kDa subunit
  • TRS120
  • Tularik gene 1 protein

Background

NIBP is a 1246 amino acid intracellular regulatory protein. Reports suggest that NIBP physically interacts with NIK, IKK (beta), but not IKK (alpha) or IKK (gamma) and thus acts as a novel enhancer of cytokine-induced NF-(kappa) B signaling pathway. Due to its neuronal expression NIBP is considered as a potential target for modulating the NF-(kappa) B signaling cascade in neuronal pathologies dependent upon abnormal activation of this pathway. NIBP is mainly expressed in brain, muscle, heart, and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional Array Products

Array NB100-55735

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Bioinformatics

Gene Symbol TRAPPC9
Entrez
Uniprot