Endothelial Lipase Antibody


Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)
Immunocytochemistry/ Immunofluorescence: Endothelial Lipase Antibody [NB400-118] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Western Blot: Endothelial Lipase Antibody [NB400-118] - Analysis of Endothelial Lipase in ECV304 cells.

Product Details

Reactivity Hu, Mu(-)Species Glossary
Applications WB, ELISA, ICC/IF
1 mg/ml

Endothelial Lipase Antibody Summary

An N-terminal synthetic peptide made to human Endothelial Lipase [UniProt# Q9Y5X9]
Secreted, cytoplasm
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.01% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
This Endothelial Lipase antibody is useful for ELISA (PMID: 22383927), Western Blot and Immunocytochemistry/Immunofluorescence. In Western blot this antibody recognizes a band at ~57 kDa, representing the mature, non-glycosylated form of endothelial lipase protein. Another band is seen at ~ 45 kDa, representing the proteolytic form of the protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells.
Control Peptide
Endothelial Lipase Peptide (NB400-118PEP)
Read Publications using
NB400-118 in the following applications:

Reactivity Notes

Does not appear to recognize mouse.

Alternate Names for Endothelial Lipase Antibody

  • EC 3.1.1
  • EC
  • EDLendothelial lipase
  • ELlipoprotein lipase H
  • Endothelial cell-derived lipase
  • lipase, endothelial
  • PRO719


Endothelial lipase (EL) is an essential element of HDL metabolism which catalyzes the hydrolysis of HDL-phospholipids for facilitating their clearance from circulation. EL is synthesized in endothelial cells, however, is also expressed in other cell types such as coronary artery endothelial cells, umbilical vein endothelial cells, hepatocytes, osteosarcoma cell lines etc. with highest levels seen in organs including liver, placenta, lung, thyroid, kidney, testis and corpus luteum of ovary. EL is synthesized as a 55 kD protein, secreted as a 68 kD mature protein and after secretion, a portion of EL is cleaved and inactivated by convertases. Uncleaved EL is bound to heparan-sulfate proteoglycans on the cell surfaces where via its bridging functions, it facilitates HDL particle binding/uptake and selective uptake of HDL cholesteryl esters. Through its marked sn-1 phospholipase activity, EL cleaves lipoprotein-associated phospholipids, primarily HDL-phosphatidylethanolamine (HDL-PE) and -phosphatidylcholine (HDL-PC), generating mostly saturated free fatty acids and sn-1 lyso sn-2 acyl lysophospholipids (LysoPL). By its rather weak lysophospholipase activity, EL further hydrolyzes some of those EL-generated LPL, liberating unsaturated FFA. EL expression increases in cultured endothelial cells exposed to interleukin-1 beta or TNF-alpha as well as in vivo upon LPS exposure suggesting its role in inflammatory conditions. Accordingly, an elevated EL levels are found in patients with subclinical inflammation as encountered in the metabolic syndrome and coronary atherosclerosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endothelial Lipase Antibody (NB400-118)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.

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Showing Publications 1 - 3 of 3.
Publications using NB400-118 Applications Species
Riederer M, Trbusi M, Degoricija V, Frank S. Endothelial Lipase Plasma Levels are Increased in Patients With Significant Carotid Artery Stenosis and History of Neurological Impairment J Clin Med Res 2012 Jan 15 [PMID:22383927] (ELISA, Human) ELISA Human
Edmondson AC, Brown RJ, Kathiresan S et al. Loss-of-function variants in endothelial lipase are a cause of elevated HDL cholesterol in humans. J Clin Invest 2009 Apr [PMID:19287092]
Riederer M, Trbusic M, Degoricija V, Frank S Endothelial Lipase Plasma Levels are Increased in Patients With Significant Carotid Artery Stenosis and History of Neurological Impairment. J Clin Med Res;4(1):49-51. 2012 Jan 15.

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Product General Protocols

View specific protocols for Endothelial Lipase Antibody (NB400-118): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endothelial Lipase Antibody (NB400-118) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB400-118AF405
Alexa Fluor (R) 488 Labeled NB400-118AF488
Alexa Fluor (R) 647 Labeled NB400-118AF647
Alexa Fluor (R) 700 Labeled NB400-118AF700
Biotin Labeled NB400-118B
DyLight 405 Labeled NB400-118V
DyLight 488 Labeled NB400-118G
DyLight 550 Labeled NB400-118R
DyLight 650 Labeled NB400-118C
FITC Labeled NB400-118F
HRP Labeled NB400-118H

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Contact Information

Product PDFs


Gene Symbol LIPG

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