Endothelial Lipase Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Endothelial Lipase Antibody [DyLight 550] Summary

Immunogen
An N-terminal synthetic peptide made to human Endothelial Lipase [UniProt# Q9Y5X9]
Localization
Secreted, cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIPG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Endothelial Lipase antibody is useful for ELISA (PMID: 22383927), Western Blot and Immunocytochemistry/Immunofluorescence. In Western blot this antibody recognizes a band at ~57 kDa, representing the mature, non-glycosylated form of endothelial lipase protein. Another band is seen at ~ 45 kDa, representing the proteolytic form of the protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells.

Reactivity Notes

Does not appear to recognize mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Endothelial Lipase Antibody [DyLight 550]

  • EC 3.1.1
  • EC 3.1.1.3
  • EDLendothelial lipase
  • ELlipoprotein lipase H
  • Endothelial cell-derived lipase
  • lipase, endothelial
  • PRO719

Background

Endothelial lipase (EL) is a novel member of the lipoprotein lipase gene family. It is synthesized by endothelial cells and functions at the site of synthesis. EL most likely plays a physiological role in modulating lipoprotein metabolism. Overexpressed EL has been shown to reduce HDL cholesterol levels, whereas an EL deficiency appears to be effective in elevating HDL cholesterol levels in animals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endothelial Lipase Antibody (NB400-118R) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Endothelial Lipase Antibody (NB400-118R)

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Bioinformatics

Gene Symbol LIPG