Endostatin Antibody (1837.46)

Immunohistochemistry-Paraffin: Endostatin Antibody (1837.46) [NBP1-40051] - Analysis of anti-COL18A1 / Endostatin antibody with human kidney at dilution 1:100.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 1837.46
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Endostatin Antibody (1837.46) Summary

• Description: Novus Biologicals Mouse Endostatin Antibody (1837.46) (NBP1-40051) is a monoclonal antibody validated for use in IHC and WB. Anti-Endostatin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length recombinant murine Endostatin.
• Specificity: An unidentified protein is also weakly detected, Mr 54kD.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL18A1
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:100-1:500
• Application Notes: .

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Sodium Phosphate (pH 8.0) and 30% Glycerol. Sourced in Ascites.
• Preservative: 0.05% Sodium Azide
• Purity: Ion exchange chromatography

Alternate Names for Endostatin Antibody (1837.46)

• antiangiogenic agent
• COL18A1
• collagen alpha-1(XVIII) chain
• collagen, type XVIII, alpha 1
• Endostatin
• FLJ27325
• FLJ34914
• human type XVIII collagen10endostatin
• KNO
• KNO1MGC74745
• Knobloch syndrome, type 1
• KS
• multi-functional protein MFP

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endostatin Antibody (NBP1-40051)(3)

Publications using NBP1-40051

• Song N, Ding Y, Zhuo W et al. The nuclear translocation of endostatin is mediated by its receptor nucleolin in endothelial cells Angiogenesis 2012-06-19 [PMID: 22711211] (WB, Human)
• Chen KH, Hsu CC, Song WS et al. Celecoxib enhances radiosensitivity in medulloblastoma-derived CD133-positive cells. Childs Nerv Syst 2010-11-01 [PMID: 20526717] (WB, Human)
• Mangieri Domenica, Nico Beatrice, Benagiano Vincenzo et al. Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. Journal of Cellular and Molecular Medicine 2008-01-01 [PMID: 18494942] (WB, Human)

Bioinformatics

• Gene Symbol: COL18A1
• Uniprot:
  • P39060()