Cytokeratin, pan Antibody (AE-1/AE-3)

Images

 
Orthogonal Strategies: Dual RNAscope ISH-IHC: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for Pan Cytokeratin mRNA ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - MCF-7 cells stained with Biotin conjugated version of pan Cytokeratin antibody and SAv-A488. A) SAv-A488 only at a dilution ...read more
Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody ...read more
Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (AE1 + AE3) [NBP2-29429] - Pan Cytokeratin was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 1.7 ug/mL of mouse ...read more
Flow (Intracellular): Cytokeratin, pan Antibody (AE1 + AE3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200R (blue) and a matched isotype control ...read more
Flow Cytometry: Cytokeratin, pan Antibody (AE1 + AE3) [NBP2-29429] - Human Pan-Cytokeratins on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (AE-1/AE-3)
Flow Cytometry: Cytokeratin, pan Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Pan-Cytokeratin Monoclonal ...read more
Flow Cytometry: Cytokeratin, pan Antibody (AE1 + AE3) [NBP2-29429] - Using the PE direct conjugate An intracellular stain was performed on HT-29 cells with pan Cytokeratin (AE1 + AE3) antibody NBP2-33200PE (blue) and a ...read more
Flow (Intracellular): Cytokeratin, pan Antibody (AE1 + AE3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200AF647 (blue) and a matched isotype ...read more
Flow (Intracellular): Cytokeratin, pan Antibody (AE1 + AE3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200PE (blue) and a matched isotype control ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, Re, ZeSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, Single-Cell Western
Clone
AE-1/AE-3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

Cytokeratin, pan Antibody (AE-1/AE-3) Summary

Immunogen
Total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
Localization
Cytoplasmic
Marker
Epithelial marker
Specificity
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2ug/ml
  • Flow Cytometry 1-2ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.25-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.25-1.0ug/ml
  • Dual RNAscope ISH-IHC
  • Flow (Intracellular)
  • Single Cell Western 1:10
Application Notes
1. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes. The staining pattern of the pan cytokeratin antibody cocktail may be different than that of either antibody separately.
2. Mol. Weight of Antigen: 40 - 67 kDa. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). The pan cytokeratin cocktail does not react with keratin18, which is also expressed in carcinomas. As such, negative staining with NBP2-29429 in of itself may not be sufficient evidence to rule out the possibility of a carcinoma (Ordonez, 2013).
a. For example, hepatocellular, adrenal cortical, clear cell renal and chromophobe renal cell carcinomas have been reported to be negative for the pan cytokeratin antibody. In this regard, the pan cytokeratin antibody can be used as part of a screening panel to more extensively define the tumor cell lineages.
3. The pan cytokeratin antibody may cross-react with GFAP, leading to aberrant positive staining of glial tumors such as ependymoma, glioblastoma, or schwannoma (Ordonez, 2013). Use in Immunohistochemistry reported in scientific literature (PMID: 29169625). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Publications
Read Publications using
NBP2-29429 in the following applications:

Reactivity Notes

Reptile reactivity reported in scientific literature (PMID: 11351328). Zebrafish reactivity reported in scientific literature (PMID: 30970016).

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Background

Keratin/cytokeratin AE1 + AE3 is a pan cytokeratin (also known as pan keratin) antibody cocktail that detects cytokeratins 1-8, 10, 14-16 and 19 (reviewed in Ordonez, 2013). The cocktail is an optimized mixture of two different monoclonal antibody clones, AE1 and AE3. Each clone detects a subset of high and low molecular weight cytokeratins: AE1 detects 10, 14-16, and 19; AE3 detects 1-8. A key advantage of the pan cytokeratin antibody cocktail stain is that a broader spectrum of cytokeratins can be detected compared to using each clone alone.
The pan cytokeratin antibody cocktail is a well known broad spectrum immunohistochemical epithelial marker for screening for epithelial differentiation in tumors and their metastases. Most carcinomas have been reported to stain positive, and the antibody can help confirm or rule out the epithelial nature of a poorly differentiated tumor. Positive pan cytokeratin staining with the antibody in the lymph node (Nikura, 2007) or bone marrow ((Berg, 2007) can be an indication of metastatic carcinoma. The pan cytokeratin antibody has also been used to identify residual tumor post-treatment (Azumi, 2006), assess the depth of cancerous invasion (Alexander-Sefre, 2004) and predict survival outcome (Wiedswang, 2004).
Negative pan cytokeratin staining patterns can suggest non-epithelial components associated with a carcinoma. For example, ductal lavage foam cells from breast carcinoma patients did not stain with the antibody (Krishnamurthy, 2002). Foam cells are apparently of macrophage origin and hence pan cytokeratin cocktail negative.
The pan cytokeratin antibody staining patterns have been extensively documented in epithelium and numerous tumor types, dating back to 1982 (Woodcock-Mitchell) when the clones were first developed. As such, researchers are encouraged to survey the published literature for additional information about pan cytokeratin positive and negative staining tumors as well as in normal tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, Single-Cell Western

Publications for Cytokeratin, pan Antibody (NBP2-29429)(21)

We have publications tested in 5 confirmed species: Human, Canine, Chicken, Lizards, Zebrafish.

We have publications tested in 6 applications: ICC, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC
(1)
ICC/IF
(5)
IHC
(2)
IHC-Fr
(1)
IHC-P
(6)
WB
(2)
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Filter By Species
Human
(5)
Canine
(1)
Chicken
(2)
Lizards
(1)
Zebrafish
(2)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NBP2-29429 Applications Species
Oppel F, Ki DH, Zimmermann MW et al. suz12 inactivation in p53 and nf1 deficient zebrafish accelerates the onset of MPNSTs and expands the spectrum of tumor types to include adenocarcinoma, leukemia, and soft tissue sarcoma Dis Model Mech Jul 10 2020 [PMID: 32651197] (ICC/IF, Zebrafish) ICC/IF Zebrafish
Liu Z, Murphy SF, Huang J et al. A novel immunocompetent model of metastatic prostate cancer-induced bone pain Prostate Jul 1 2020 [PMID: 32407603]
Estermann MA, Williams S, Hirst CE et al. Insights into Gonadal Sex Differentiation Provided by Single-Cell Transcriptomics in the Chicken Embryo Cell Rep Apr 7 2020 [PMID: 32268081] (IHC-Fr, Chicken) IHC-Fr Chicken
Fukushima K, Satoh T, Sugihara F et Al. Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis Immunity. [PMID: 32187520]
Dey P, Li J, Zhang J et al. Oncogenic Kras driven metabolic reprogramming in pancreas cancer cells utilizes cytokines from the tumor microenvironment Cancer Discov Feb 11 2020 [PMID: 32046984] (IHC-P, Human) IHC-P Human
Roly ZY, Major AT, Fulcher A, et al. Adhesion G-protein coupled receptor, GPR56, is required for MUllerian duct development in the chick J. Endocrinol. Dec 1 2019 [PMID: 31829965] (IHC, Chicken) IHC Chicken
Oppel F, Tao T, Shi H et al. Loss of atrx cooperates with p53-deficiency to promote the development of sarcomas and other malignancies. PLoS Genet. 2019 Apr 10 [PMID: 30970016] (IHC-P, Zebrafish)

Details:
Citation using the Azide and BSA Free form of this antibody.
IHC-P Zebrafish
Wan J, Block S, Scribano CM et al. Mad1 destabilizes p53 by preventing PML from sequestering MDM2. Nat Commun. 2019 Apr 4 [PMID: 30948704] (IHC-P, Human)

Details:
Citation using the Azide and BSA Free form of this antibody.
IHC-P Human
Jensen T, Wanczyk H, Sharma I et al. Polyurethane scaffolds seeded with autologous cells can regenerate long esophageal gaps: An esophageal atresia treatment model. J Pediatr Surg. 2018 Oct 22 [PMID: 30429066] (ICC/IF)

Details:
Citation using the Azide and BSA Free form of this antibody.
ICC/IF
Ijsselsteijn ME, Brouwer TP, Abdulrahman Z et al. Cancer immunophenotyping by seven-colour multispectral imaging without tyramide signal amplification. J Pathol Clin Res. 2018 Dec 4 [PMID: 30191683] (ICC/IF, Human)

Details:
Citation using the Alexa Fluor 647 form of this antibody.
ICC/IF Human
Show All 21 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin, pan Antibody (NBP2-29429). (Showing 1 - 1 of 1 FAQs).

  1. Could you tell us recommended antigen retrieval method for this antibody?-EndFragment-->
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution of antibody for IHC should be determined by the user. 

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33200AF350
Alexa Fluor 405 NBP2-33200AF405
Alexa Fluor 488 NBP2-33200AF488
Alexa Fluor 532 NBP2-33200AF532
Alexa Fluor 594 NBP2-33200AF594
Alexa Fluor 647 NBP2-33200AF647
Alexa Fluor 700 NBP2-33200AF700
Alexa Fluor 750 NBP2-33200AF750
Allophycocyanin NBP2-33200APC
Biotin NBP2-33200B
DyLight 350 NBP2-33200UV
DyLight 405 NBP2-33200V
DyLight 488 NBP2-33200G
DyLight 550 NBP2-33200R
DyLight 594 NBP2-33200DL594
DyLight 650 NBP2-33200C
DyLight 680 NBP2-33200FR
DyLight 755 NBP2-33200IR
FITC NBP2-33200F
HRP NBP2-33200H
Janelia Fluor 549 NBP2-33200JF549
Janelia Fluor 646 NBP2-33200JF646
PE NBP2-33200PE
PerCP NBP2-33200PCP

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Bioinformatics

Gene Symbol KRT1