Endophilin B1/Bif-1 Antibody (30A882.1.1)


Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate.
Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Simple Western lane view shows a specific band for Endophilin B1/Bif-1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
0.5 mg/ml

Endophilin B1/Bif-1 Antibody (30A882.1.1) Summary

A full-length human Bif-1 recombinant protein was used as immunogen.
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
100 ug in 200 ul PBS containing 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


  • Western Blot 1-2 ug/ml
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point. Use in Immunocytochemistry/immunofluorescence reported in multiple pieces of scientific literature
Read Publications using
NBP2-24733 in the following applications:

  • 3 publications
  • IP
    1 publication
  • WB
    6 publications

Reactivity Notes

This antibody is reactive to Human. Mouse reactivity reported in scientific literature (PMID: 25619828)

Alternate Names for Endophilin B1/Bif-1 Antibody (30A882.1.1)

  • Bax-interacting factor 1
  • Bif1
  • Bif-1
  • Bif-1dJ612B15.2
  • CGI-61
  • endophilin-B1
  • KIAA0491endophilin B1
  • SH3 domain-containing GRB2-like protein B1
  • SH3-containing protein SH3GLB1
  • SH3-domain GRB2-like endophilin B1
  • SH3-domain, GRB2-like, endophilin B1
  • SH3GLB1


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, IP
Species: Hu, Mu, Rt
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Publications for Endophilin B1/Bif-1 Antibody (NBP2-24733)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP2-24733 Applications Species
Wang YH, Wang JQ, Wang Q et al. Endophilin B2 promotes inner mitochondrial membrane degradation by forming heterodimers with Endophilin B1 during mitophagy. Sci Rep. 2016 Apr 26 [PMID:27112121]
Xu S, Cherok E, Das S et al. Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein. Mol. Biol. Cell. 2015 Nov 12 [PMID:26564796] (WB, Human) WB Human
Wang DB, Kinoshita Y, Kinoshita C et al. Loss of endophilin-B1 exacerbates Alzheimer's disease pathology. Brain. 2015 May 16 [PMID:25981964] (WB, Mouse, Human) WB Mouse, Human
Dong H, Tian L, Li R et al. IFNg-induced Irgm1 promotes tumorigenesis of melanoma via dual regulation of apoptosis and Bif-1-dependent autophagy Oncogene. 2015 Jan 26 [PMID:25619828] (WB, Mouse) WB Mouse
Wang DB, Uo T, Kinoshita C et al. Bax interacting factor-1 promotes survival and mitochondrial elongation in neurons. J. Neurosci. 2014 Feb 12 [PMID:24523556] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Runkle Kristin B, Meyerkord Cheryl L, Desai Neelam V et al. Bif-1 suppresses breast cancer cell migration by promoting EGFR endocytic degradation. Cancer Biol Ther. 2012 Aug [PMID:22785202] (Human) Human
Cant Jonathan S, Goodale Melvyn A. Scratching beneath the surface: new insights into the functional properties of the lateral occipital area and parahippocampal place area. J Neurosci. 2011 Jun 1 [PMID:21632946] (ICC/IF, Human) ICC/IF Human
Takahashi Yoshinori, Meyerkord Cheryl L, Hori Tsukasa et al. Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy. 2011 Jan [PMID:21068542] (ICC/IF) ICC/IF
Yang J, Takahashi Y, Cheng E et al. GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death. J Cell Sci. 2010 Mar 15 [PMID:20159967] (WB) WB
Etxebarria A, Terrones O, Yamaguchi H et al. Endophilin B1/Bif-1 stimulates BAX activation independently from its capacity to produce large scale membrane morphological rearrangements. J Biol Chem. 2009 Feb 13 [PMID:19074440] (WB, Rat) WB Rat
Show All 15 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP2-24733AF405
Alexa Fluor (R) 488 Labeled NBP2-24733AF488
Alexa Fluor (R) 647 Labeled NBP2-24733AF647
Alexa Fluor (R) 700 Labeled NBP2-24733AF700
Allophycocyanin Labeled NBP2-24733APC
Biotin Labeled NBP2-24733B
DyLight 350 Labeled NBP2-24733UV
DyLight 405 Labeled NBP2-24733V
DyLight 405LS Labeled NBP2-24733V3
DyLight 488 Labeled NBP2-24733G
DyLight 550 Labeled NBP2-24733R
DyLight 650 Labeled NBP2-24733C
DyLight 680 Labeled NBP2-24733FR
DyLight 755 Labeled NBP2-24733IR
HRP Labeled NBP2-24733H
PE Labeled NBP2-24733PE
PerCP Labeled NBP2-24733PCP

Additional Endophilin B1/Bif-1 Products

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Diseases for Endophilin B1/Bif-1 Antibody (NBP2-24733)

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PTMs for Endophilin B1/Bif-1 Antibody (NBP2-24733)

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Contact Information

Product PDFs


Gene Symbol SH3GLB1

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