Endophilin B1/Bif-1 Antibody (30A882.1.1)

Images

 
Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate.
Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 5 ug/ml conc. of Endophilin B1 antibody (clone ...read more
Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Simple Western lane view shows a specific band for Endophilin B1/Bif-1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clone
30A882.1.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Endophilin B1/Bif-1 Antibody (30A882.1.1) Summary

Immunogen
A full-length human Bif-1 recombinant protein was used as immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SH3GLB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in multiple pieces of scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publications using
NBP2-24733 in the following applications:

  • 4 publications
  • IHC
    1 publication
  • IP
    2 publications
  • WB
    11 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25619828)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Endophilin B1/Bif-1 Antibody (30A882.1.1)

  • Bax-interacting factor 1
  • Bif1
  • Bif-1
  • Bif-1dJ612B15.2
  • CGI-61
  • Endophilin B1
  • endophilin-B1
  • KIAA0491endophilin B1
  • SH3 domain-containing GRB2-like protein B1
  • SH3-containing protein SH3GLB1
  • SH3-domain GRB2-like endophilin B1
  • SH3-domain, GRB2-like, endophilin B1
  • SH3GLB1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endophilin B1/Bif-1 Antibody (NBP2-24733)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(4)
IHC
(1)
IP
(2)
WB
(11)
All Applications
Filter By Species
Human
(9)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NBP2-24733 Applications Species
Wang DB, kinoshita C, kinoshita Y et al. Neuronal Susceptibility to Beta-Amyloid Toxicity and Ischemic Injury Involves Histone Deacetylase-2 Regulation of Endophilin-B1. Brain Pathol. Jul 20 2018 [PMID: 30028551] (WB, Mouse) WB Mouse
Serfass JM, Takahashi Y, Zhou Z et al. Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection J. Biol. Chem. Jun 16 2017 [PMID: 28455444] (WB, ICC/IF, Human) WB, ICC/IF Human
Marcassa E, Raimondi M, Anwar T et al. Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin. Biol Open. Mar 16 2017 [PMID: 28302665] (WB, Human) WB Human
Zhang C, Liu F, Chen H et al. Bif-1 promotes tumor cell migration and metastasis via Cdc42 expression and activity. Clin. Exp. Metastasis Oct 11 2016 [PMID: 27730394] (IHC, Human) IHC Human
Wild F, Khan MM, Straka T, Rudolf R. Progress of endocytic CHRN to autophagic degradation is regulated by RAB5-GTPase and T145 phosphorylation of SH3GLB1 at mouse neuromuscular junctions in vivo. Autophagy Oct 7 2016 [PMID: 27715385] (WB, Mouse) WB Mouse
Wang YH, Wang JQ, Wang Q et al. Endophilin B2 promotes inner mitochondrial membrane degradation by forming heterodimers with Endophilin B1 during mitophagy. Sci Rep. 2016 Apr 26 [PMID: 27112121]
Takahashi Y, Tsotakos N, Liu Y et al. The Bif-1-Dynamin 2 membrane fission machinery regulates Atg9-containing vesicle generation at the Rab11-positive reservoirs. Oncotarget. Apr 12 2016 [PMID: 26980706] (IP, WB, Human) IP, WB Human
Xu S, Cherok E, Das S et al. Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein. Mol. Biol. Cell. 2015 Nov 12 [PMID: 26564796] (WB, Human) WB Human
Wang DB, Kinoshita Y, Kinoshita C et al. Loss of endophilin-B1 exacerbates Alzheimer's disease pathology. Brain. 2015 May 16 [PMID: 25981964] (WB, Mouse, Human)

Details:
Endophilin B1/Bif-1 antibody (clone 30A882.1.1) used for WB application at 1:500 dilution in experiments involving a mouse model of Alzheimer’s disease pathology. Figure 1 shows the expression of Endophilin B1/Bif-1 in lysates from hippocampus and cortex of 10-month-old double mutant amyloid precursor protein and presenilin 1 (APPswe/PSEN1dE9) mouse model of Alzheimer’s disease and wild-type littermates. WB also performed on postnatal cortical neurons which were infected with EGFP or endophilin-B1 isoforms for 3 days, then treated with 10 mM amyloid-b25-35 or reverse peptide amyloid-b35-25 for 24 h (Figure 6A). Expression of Endophilin B1 was also studied in the inferior parietal lobule cerebral cortex of patients at various Braak stages (Figure 7). In all WB results, this antibody generated two major bands with the lower band representing ubiquitously expressed endophilin-B1a isoform and the upper band corresponding to neuron-specific isoforms, endophilin-B1b/c (described previously also by Wang et al., 2014; PMID 24523556).
WB Mouse, Human
Dong H, Tian L, Li R et al. IFNg-induced Irgm1 promotes tumorigenesis of melanoma via dual regulation of apoptosis and Bif-1-dependent autophagy Oncogene. 2015 Jan 26 [PMID: 25619828] (WB, Mouse)

Details:
Bif-1/Endophilin-B1 / SH3GLB1 antibody used for WB assay on lysates of Irgm1-overexpressing B16 cells that were transfected with control siRNA or Bif-1-siRNA followed by 6 or 24 hours of starvation with serum-deprived low-glucose conditioned medium treatment (Figure 7a).
WB Mouse
Show All 21 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-24733AF405
Alexa Fluor 488 Labeled NBP2-24733AF488
Alexa Fluor 647 Labeled NBP2-24733AF647
Alexa Fluor 700 Labeled NBP2-24733AF700
Biotin Labeled NBP2-24733B
DyLight 350 Labeled NBP2-24733UV
DyLight 405 Labeled NBP2-24733V
DyLight 488 Labeled NBP2-24733G
DyLight 550 Labeled NBP2-24733R
DyLight 650 Labeled NBP2-24733C
DyLight 680 Labeled NBP2-24733FR
DyLight 755 Labeled NBP2-24733IR
HRP Labeled NBP2-24733H

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Bioinformatics

Gene Symbol SH3GLB1
Entrez
Uniprot