Endoglin/CD105 Antibody [Unconjugated]

Western blot shows lysates of rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6440) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Endoglin/CD105 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Rat Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the transmembrane. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Endoglin/CD105 was detected in perfusion fixed frozen sections of rat kidney using Goat Anti-Rat Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6440) at 1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells in glomeruli. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Endoglin/CD105 was immunoprecipitated from 400 µg of rat embryo tissue lysates using 4 µg of Goat Anti-Rat Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6440) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Rat embryo tissue lysates or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Endoglin/CD105 was detected by Western blot under reducing conditions using 1 µg/mL Goat Anti-Rat Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6440) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.

• Reactivity: Rt
• Applications: WB, IHC, IP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Endoglin/CD105 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Endoglin/CD105
  Met1-Gly578
  Accession # Q6Q3E8
• Specificity: Detects rat Endoglin/CD105 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross reactivity with recombinant mouse Endoglin is observed and approximately 50% cross reactivity with recombinant human Endoglin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ENG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endoglin/CD105 Antibody [Unconjugated]

• CD105 antigen
• CD105
• Endoglin
• ENDOsler-Rendu-Weber syndrome 1
• ENG
• HHT1FLJ41744
• ORW
• ORW1

Background

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin and betaglycan/T beta RIII are type III receptors for TGF-beta superfamily ligands, sharing 71% aa identity with the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells. Rat Endoglin cDNA encodes 650 amino acids (aa) including a 25 aa signal sequence, a 553 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 51 aa cytoplasmic domain. In human and mouse, an isoform with a 14 aa cytoplasmic domain (S‑endoglin) can oppose effects of long (L) Endoglin. In rat, a potential isoform with a 100 aa cytoplasmic tail (49 aa inserted after aa 610) diverges at the same aa as S‑endoglin. The rat Endoglin ECD shares 84%, 70%, 68%, 64%, and 62% aa identity with mouse, human, canine, porcine, and bovine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII. Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6. BMP-9, however, is reported to bind Endoglin directly. Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line. In endothelial cells, Endoglin inhibits T beta RI/ALK-5, but enhances ALK‑1‑mediated activation. Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization. In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to anti-angiogenic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endoglin/CD105 Antibody (AF6440)(2)

Publications using AF6440

• Yihui Yang, Hongfu Yu, Congwen Yang, Yunfei Zhang, Xiangfa Ai, Xiaobo Wang et al. Krüppel-like factor 6 (KLF6) mediates pulmonary angiogenesis in rat experimental hepatopulmonary syndrome and is aggravated by bone morphogenetic protein 9 (BMP9) Biology Open 2019-06-26 [PMID: 31189661]
• Ming Gong, Sahaya Antony, Reiko Sakurai, Jie Liu, Michelina Iacovino, Virender K. Rehan Bone marrow mesenchymal stem cells of the intrauterine growth-restricted rat offspring exhibit enhanced adipogenic phenotype International Journal of Obesity 2016-11-01 [PMID: 27599633]

FAQs for Endoglin/CD105 Antibody (AF6440). (Showing 1 - 2 of 2 FAQs).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: ENG
• Uniprot:
  • Q6Q3E8()