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Endoglin/CD105 Antibody (ENG/1327)

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Western Blot: Endoglin/CD105 Antibody (ENG/1327) [NBP2-53334] - Western Blot of HeLa and A431 Cell Lysates with Endoglin / CD105 Monoclonal Antibody (ENG/1327).
Western Blot: Endoglin/CD105 Antibody (ENG/1327) [NBP2-53334] - Western Blot of HeLa and A431 Cell Lysates with Endoglin / CD105 Monoclonal Antibody (ENG/1327).
SDS-Page: Endoglin/CD105 Antibody (ENG/1327) [NBP2-53334]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, PAGE
Clone
ENG/1327
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54517AF350
Alexa Fluor 405
NBP2-54517AF405
Alexa Fluor 488
NBP2-54517AF488
Alexa Fluor 532
NBP2-54517AF532
Alexa Fluor 594
NBP2-54517AF594
Alexa Fluor 647
NBP2-54517AF647
Alexa Fluor 700
NBP2-54517AF700
Alexa Fluor 750
NBP2-54517AF750
Allophycocyanin
NBP2-54517APC
Biotin
NBP2-54517B
DyLight 350
NBP2-54517UV
DyLight 405
NBP2-54517V
DyLight 488
NBP2-54517G
DyLight 550
NBP2-54517R
DyLight 594
NBP2-54517DL594
DyLight 650
NBP2-54517C
DyLight 680
NBP2-54517FR
DyLight 755
NBP2-54517IR
FITC
NBP2-54517F
HRP
NBP2-54517H
Janelia Fluor 549
NBP2-54517JF549
Janelia Fluor 646
NBP2-54517JF646
PE
NBP2-54517PE
PerCP
NBP2-54517PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-54517-100ug
100% Guarantee on every product

Endoglin/CD105 Antibody (ENG/1327) Summary

Immunogen
Recombinant human Endoglin protein fragment (aa74-251) (exact sequence is proprietary)
Localization
Cytoplasmic
Marker
Angiogenesis Marker
Protein/Peptide Type
Recombinant Protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ENG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • SDS-Page
Application Notes
Human chromosome location: 9q34.11
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Endoglin/CD105 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Endoglin/CD105 Antibody (ENG/1327)

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.
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Publications for Endoglin/CD105 Antibody (NBP2-53334) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endoglin/CD105 Antibody (NBP2-53334). (Showing 1 - 2 of 2 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Control Lysate(s)

Endoglin/CD105 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-54517AF350
Alexa Fluor 405 NBP2-54517AF405
Alexa Fluor 488 NBP2-54517AF488
Alexa Fluor 532 NBP2-54517AF532
Alexa Fluor 594 NBP2-54517AF594
Alexa Fluor 647 NBP2-54517AF647
Alexa Fluor 700 NBP2-54517AF700
Alexa Fluor 750 NBP2-54517AF750
Allophycocyanin NBP2-54517APC
Biotin NBP2-54517B
DyLight 350 NBP2-54517UV
DyLight 405 NBP2-54517V
DyLight 488 NBP2-54517G
DyLight 550 NBP2-54517R
DyLight 594 NBP2-54517DL594
DyLight 650 NBP2-54517C
DyLight 680 NBP2-54517FR
DyLight 755 NBP2-54517IR
FITC NBP2-54517F
HRP NBP2-54517H
Janelia Fluor 549 NBP2-54517JF549
Janelia Fluor 646 NBP2-54517JF646
PE NBP2-54517PE
PerCP NBP2-54517PCP
Learn about Custom Antibody Labeling

Additional Endoglin/CD105 Products

Bioinformatics Tool for Endoglin/CD105 Antibody (NBP2-53334)

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Bioinformatics

Gene Symbol ENG