Endoglin/CD105 Antibody (166707) [Fluorescein]

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB10971F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 166707
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

Endoglin/CD105 Antibody (166707) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105
  Glu26-Gly586
  Accession # Q5T9B9
• Specificity: Detects human Endoglin/CD105.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endoglin/CD105 Antibody (166707) [Fluorescein]

• CD105 antigen
• CD105
• Endoglin
• ENDOsler-Rendu-Weber syndrome 1
• ENG
• HHT1FLJ41744
• ORW
• ORW1

Background

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endoglin/CD105 Antibody (FAB10971F)(18)

Publications using FAB10971F

• JY Kim, S Park, HJ Lee, H Lew, GJ Kim Functionally enhanced placenta-derived mesenchymal stem cells inhibit adipogenesis in orbital fibroblasts with Graves' ophthalmopathy Stem Cell Res Ther, 2020-11-05;11(1):469. 2020-11-05 [PMID: 33153489] (Flow Cytometry, Human)
• JM Sierra-Par, A Merino, M Eijken, H Leuvenink, R Ploeg, BK Møller, B Jespersen, CC Baan, MJ Hoogduijn Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury Stem Cell Res Ther, 2020-08-12;11(1):352. 2020-08-12 [PMID: 32787906] (Flow Cytometry, Immunophenotyping, Human)
• Chantal Voskamp, Wendy J. L. M. Koevoet, Rodrigo A. Somoza, Arnold I. Caplan, Véronique Lefebvre, Gerjo J. V. M. van Osch et al. Enhanced Chondrogenic Capacity of Mesenchymal Stem Cells After TNF alpha Pre-treatment Frontiers in Bioengineering and Biotechnology 2020-06-30 [PMID: 32714905]
• MR Mahmoudian, F Forouzanfa, S Asgharzade, N Ghorbani Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture J Ophthalmol, 2019-12-11;2019(0):2454362. 2019-12-11 [PMID: 31885884] (Flow Cytometry, Human)
• L de Girolam, H Schönhuber, M Viganò, C Bait, A Quaglia, G Thiebat, P Volpi Autologous Matrix-Induced Chondrogenesis (AMIC) and AMIC Enhanced by Autologous Concentrated Bone Marrow Aspirate (BMAC) Allow for Stable Clinical and Functional Improvements at up to 9 Years Follow-Up: Results from a Randomized Controlled Study J Clin Med, 2019-03-21;8(3):. 2019-03-21 [PMID: 30901900] (Flow Cytometry, Human)
• S Kandoi, PK L, B Patra, P Vidyasekar, D Sivanesan, V S, R K, RS Verma Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs Sci Rep, 2018-08-20;8(1):12439. 2018-08-20 [PMID: 30127445] (Flow Cytometry, Human)
• SFH de Witte, AM Merino, M Franquesa, T Strini, JAA van Zoggel, SS Korevaar, F Luk, M Gargesha, L O'Flynn, D Roy, SJ Elliman, PN Newsome, CC Baan, MJ Hoogduijn Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease Stem Cell Res Ther, 2017-06-08;8(1):140. 2017-06-08 [PMID: 28595619] (Flow Cytometry, Human)
• de Witte S, Lambert E, Merino A, Strini T, Douben H, O'Flynn L, Elliman S, de Klein A, Newsome P, Baan C, Hoogduijn M Aging of bone marrow- and umbilical cord-derived mesenchymal stromal cells during expansion. Cytotherapy, 2017-04-24;19(7):798-807. 2017-04-24 [PMID: 28462821] (Flow Cytometry, Human)
• R Núñez-Told, E Martínez-S, C Gil-Recio, MÁ Carrasco, A Al Madhoun, S Montori, M Atari Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation BMC Cell Biol., 2017-04-21;18(1):21. 2017-04-21 [PMID: 28427322] (Flow Cytometry, Human)
• Sergio Perez-Diaz, Beatriz Garcia-Rodriguez, Yolanda Gonzalez-Irazabal, Monica Valero, Javier Lagos-Lizan, Jose M. Arbones-Mainar Knockdown of PTRF ameliorates adipocyte differentiation and functionality of human mesenchymal stem cells American Journal of Physiology-Cell Physiology 2017-01-01 [PMID: 27856429]
• Croes M, Oner F, Kruyt M, Blokhuis T, Bastian O, Dhert W, Alblas J Proinflammatory Mediators Enhance the Osteogenesis of Human Mesenchymal Stem Cells after Lineage Commitment. PLoS ONE, 2015-07-15;10(7):e0132781. 2015-07-15 [PMID: 26176237] (Flow Cytometry, Human)
• Narcisi R, Cleary M, Brama P, Hoogduijn M, Tuysuz N, ten Berge D, van Osch G Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation. Stem Cell Reports, 2015-02-26;4(3):459-72. 2015-02-26 [PMID: 25733021] (Flow Cytometry, Human)
• Berlier J, Rigutto S, Dalla Valle A, Lechanteur J, Soyfoo M, Gangji V, Rasschaert J Adenosine triphosphate prevents serum deprivation-induced apoptosis in human mesenchymal stem cells via activation of the MAPK signaling pathways. Stem Cells, 2015-01-01;33(1):211-8. 2015-01-01 [PMID: 25183652] (Flow Cytometry, Human)
• Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis. Stem Cells Dev, 2013-12-04;23(5):541-54. 2013-12-04 [PMID: 24172175] (Flow Cytometry, Human)
• Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium. PLoS ONE, 2012-10-09;7(10):e47187. 2012-10-09 [PMID: 23056608] (Flow Cytometry, Human)
• Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A Human dermis harbors distinct mesenchymal stromal cell subsets. J. Invest. Dermatol., 2011-11-03;132(3):563-74. 2011-11-03 [PMID: 22048731] (Flow Cytometry, Human)
• Crop MJ, Baan CC, Korevaar SS, Ijzermans JN, Alwayn IP, Weimar W, Hoogduijn MJ Donor-derived mesenchymal stem cells suppress alloreactivity of kidney transplant patients. Transplantation, 2009-03-27;87(6):896-906. 2009-03-27 [PMID: 19300194] (Flow Cytometry, Human)
• Zhang Y, Fisher N, Newey SE The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro. Stem Cells Dev., 2009-03-01;18(2):359-75. 2009-03-01 [PMID: 18662129] (Flow Cytometry, Human)

Review for Endoglin/CD105 Antibody (FAB10971F) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/21/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 20682625
• Species: Human

FAQs for Endoglin/CD105 Antibody (FAB10971F). (Showing 1 - 2 of 2 FAQs).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: ENG
• Uniprot:
  • Q5T9B9()