Endocan/ESM-1 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Endocan/ESM-1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Endocan/ESM-1 (R&D Systems, Catalog # 1999-EC)
  Trp20-Arg184
  Accession # Q9QYY7
• Specificity: Detects mouse Endocan/ESM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human Endocan is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ESM1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Endocan/ESM-1 Antibody (Catalog # MAB1999)
  ELISA Detection: Mouse Endocan/ESM-1 Biotinylated Antibody (Catalog # BAF1999)
  Standard: Recombinant Mouse Endocan/ESM-1 (Catalog # 1999-EC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endocan/ESM-1 Antibody [Biotin]

• Endocan
• endothelial cell-specific molecule 1
• ESM1
• ESM-1
• IGFBP-rp6

Background

Endocan (endothelial cell proteoglycan; also known as endothelial-cell specific molecule-1 [ESM-1]), is a 50 kDa, monomeric, secreted, cysteine-rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4-O sulfated N-acetyl galactosamine with alpha -iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF-alpha and VEGF, (1, 7) and is known to bind to LFA-1 ( alpha _L beta ₂) on the surface of PBMCs, blocking LFA-1 interaction with ICAM-1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).

1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
2. Lassalle, P. (1999) Genbank Accession #: Q9QYY7.
3. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
4. Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159.
5. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217
6. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
7. Tsai, J.C. et al. (2002) J. Vasc. Res. 39:148.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESM1
• Uniprot:
  • Q9QYY7()