Endocan/ESM-1 Products

NL001) and counterstained with DAPI (blue). Specific staining was localized to the retina. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">

1999-EC) was serially diluted and captured by Rat Anti-Mouse Endocan/ESM‑1 Monoclonal Antibody (Catalog # MAB1999) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

HAF017). A specific band was detected for Endocan/ESM-1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">

1810-EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Endocan/ESM-1 (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox" target="_blank">