Endocan/ESM-1 Antibody (6D4) - Azide and BSA Free

Western Blot: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Analysis of ESM1 expression in transfected 293T cell line by ESM1 monoclonal antibody (M02), clone 6D4.Lane 1: ESM1 transfected lysate(20.1 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Analysis of monoclonal antibody to ESM1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.

ELISA: Endocan/ESM-1 Antibody (6D4) [H00011082-M02] - Detection limit for recombinant GST tagged ESM1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Endocan/ESM-1 Antibody (6D4) - Azide and BSA Free Summary

• Immunogen: ESM1 (NP_008967, 85 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSNGEDPFGEEFGICKDCPYGTFGMDCRETCNCQSGICDRGTGKCLKFPFFQYSVTKSSNRFVSLTEHDMASGDGNIVREEVVKENAAGSPVMRKWLNPR
• Specificity: ESM1 - endothelial cell-specific molecule 1
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Endocan/ESM-1 Antibody (6D4) - Azide and BSA Free

• Endocan
• endothelial cell-specific molecule 1
• ESM1
• ESM-1
• IGFBP-rp6

Background

This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endocan/ESM-1 Antibody (H00011082-M02)(5)

Publications using H00011082-M02

• Scuruchi, M;Aliqu�, F;Avenoso, A;Mandraffino, G;Vermiglio, G;Minuti, A;Campo, S;Campo, GM;D'Ascola, A; Endocan Knockdown Down-Regulates the Expression of Angiogenesis-Associated Genes in Il-1� Activated Chondrocytes Biomolecules 2023-05-18 [PMID: 37238720]
• Lai CY, Chen CM, Hsu WH et al. Overexpression of Endothelial Cell-Specific Molecule 1 Correlates with Gleason Score and Expression of Androgen Receptor in Prostate Carcinoma. Int J Med Sci 2017-09-30 [PMID: 29104483]
• Kang YH, Ji NY, Lee CI et al. ESM-1 silencing decreased cell survival, migration, and invasion and modulated cell cycle progression in hepatocellular carcinoma. Amino Acids. 2010-09-07 [PMID: 20821239]
• Ji NY, Kim YH, Jang YJ et al. Identification of endothelial cell-specific molecule-1 as a potential serum marker for colorectal cancer. Cancer Sci. 2010-07-30 [PMID: 20735430]
• Liu N, Zhang LH, Du H et al. Overexpression of Endothelial Cell Specific Molecule-1 (ESM-1) in Gastric Cancer. Ann Surg Oncol. 2010-04-10 [PMID: 20383661]

Bioinformatics

• Gene Symbol: ESM1
• Entrez:
  • 11082(Human)
• OMIM:
  • 601521(Human)
• Uniprot:
  • NP_008967()