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SOS1 Antibody (SOS-01)

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Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt ...read more
Immunocytochemistry/ Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and ...read more
Immunocytochemistry/ Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clone
SOS-01
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-68800AF350
Alexa Fluor 405
NB110-68800AF405
Alexa Fluor 488
NB110-68800AF488
Alexa Fluor 532
NB110-68800AF532
Alexa Fluor 594
NB110-68800AF594
Alexa Fluor 647
NB110-68800AF647
Alexa Fluor 700
NB110-68800AF700
Alexa Fluor 750
NB110-68800AF750
DyLight 350
NB110-68800UV
DyLight 405
NB110-68800V
DyLight 488
NB110-68800G
DyLight 550
NB110-68800R
DyLight 594
NB110-68800DL594
DyLight 650
NB110-68800C
DyLight 680
NB110-68800FR
DyLight 755
NB110-68800IR
Janelia Fluor 549
NB110-68800JF549
Janelia Fluor 646
NB110-68800JF646
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SOS1 Antibody (SOS-01) Summary

Immunogen
Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein (NM_005633.3)
Specificity
The SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SOS1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
WB: Use under reducing conditions. Antibody purity is > 95% (by SDS-PAGE).
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for SOS1 Antibody (SOS-01)

  • GF1GGF1
  • GINGF
  • guanine nucleotide exchange factor
  • HGFhereditary, 1
  • NS4
  • son of sevenless homolog 1 (Drosophila)
  • son of sevenless homolog 1
  • SOS-1

Background

The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SOS1 Antibody
(SOS-01)
NB110-68800
SOS1 Antibody (SOS-01)
Species: Hu, Mu
Applications: WB, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-68800AF350
Alexa Fluor 405 NB110-68800AF405
Alexa Fluor 488 NB110-68800AF488
Alexa Fluor 532 NB110-68800AF532
Alexa Fluor 594 NB110-68800AF594
Alexa Fluor 647 NB110-68800AF647
Alexa Fluor 700 NB110-68800AF700
Alexa Fluor 750 NB110-68800AF750
DyLight 350 NB110-68800UV
DyLight 405 NB110-68800V
DyLight 488 NB110-68800G
DyLight 550 NB110-68800R
DyLight 594 NB110-68800DL594
DyLight 650 NB110-68800C
DyLight 680 NB110-68800FR
DyLight 755 NB110-68800IR
Janelia Fluor 549 NB110-68800JF549
Janelia Fluor 646 NB110-68800JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol SOS1
Entrez
Uniprot