SOS1 Antibody (SOS-01)

Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line

Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: SOS-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SOS1 Antibody (SOS-01) Summary

• Immunogen: Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein (NM_005633.3)
• Specificity: The SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
• Application Notes: WB: Use under reducing conditions. Antibody purity is > 95% (by SDS-PAGE).
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for SOS1 Antibody (SOS-01)

• GF1GGF1
• GINGF
• guanine nucleotide exchange factor
• HGFhereditary, 1
• NS4
• son of sevenless homolog 1 (Drosophila)
• son of sevenless homolog 1
• SOS-1

Background

The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOS1
• Entrez:
  • 6654(Human)
  • 20662(Mouse)
• Uniprot:
  • Q07889 (Human)