eIF4G1 [p Ser1232] Antibody

Images

 
Western Blot: eIF4G1 [p Ser1232] Antibody [NBP1-04965] - Western blot analysis of extracts from 293 cell using eIF4G Antibody (Lane 1 and 2) and eIF4G (phospho-Ser1232) antibody.
Immunohistochemistry: eIF4G1 [p Ser1232] Antibody [NBP1-04965] - eIF4G1 [p Ser1232] Antibody Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (phospho- Ser1232) antibody
Western Blot: eIF4G1 [p Ser1232] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke ...read more
Western Blot: eIF4G1 [p Ser1232] Antibody [NBP1-04965] - HT29 cells untreated or treated with Anisomycin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

eIF4G1 [p Ser1232] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human eIF4G1 [p Ser1232] around the phosphorylation site of serine 1232 (P-V-Sp-P-L).
Modification
p Ser1232
Specificity
eIF4G (phospho-Ser1232) detects endogenous levels of eIF4G only when phosphorylated at serine 1232.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4G1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-04965 in the following applications:

Publications
Read Publications using
NBP1-04965 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF4G1 [p Ser1232] Antibody

  • DKFZp686A1451
  • EIF4F
  • eIF-4G 1
  • eIF4G1
  • EIF-4G1
  • eIF-4-gamma 1
  • EIF4-gamma
  • EIF4GI
  • EIF4Gp220
  • eucaryotic translation initiation factor 4G
  • eukaryotic translation initiation factor 4 gamma 1
  • eukaryotic translation initiation factor 4 gamma, 1
  • P220

Background

eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NB200-157
Species: Hu
Applications: WB, IHC, IHC-P, KO
NBP2-24632
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB, IHC-P
NB100-240
Species: Hu, Rt
Applications: WB, IHC, IP, ICC
NBP2-24529
Species: Hu, Mu, Ca, Ch, Pm
Applications: WB, Simple Western, IHC-P
NB100-1595
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NB120-6125
Species: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
NBP1-85311
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP1-87799
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-93308
Species: Hu
Applications: WB, IP
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP2-16309
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
H00008569-M14
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P
NB600-1049
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P
H00008894-M09
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NBP2-67627
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-16293
Species: Hu
Applications: WB, ICC/IF
NBP1-04965
Species: Hu
Applications: WB, IHC, IHC-P

Publications for eIF4G1 Antibody (NBP1-04965)(3)

Review for eIF4G1 Antibody (NBP1-04965) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP1-04965:
Filter by Applications
WB
(1)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Western Blot eIF4G1 NBP1-04965
Enlarge
reviewed by:
Anonymous
WB 01/11/2013
View

Summary

ApplicationWestern Blot

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for eIF4G1 Antibody (NBP1-04965) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional eIF4G1 Products

Bioinformatics Tool for eIF4G1 Antibody (NBP1-04965)

Discover related pathways, diseases and genes to eIF4G1 Antibody (NBP1-04965). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for eIF4G1 Antibody (NBP1-04965)

Discover more about diseases related to eIF4G1 Antibody (NBP1-04965).
 

Pathways for eIF4G1 Antibody (NBP1-04965)

View related products by pathway.

PTMs for eIF4G1 Antibody (NBP1-04965)

Learn more about PTMs related to eIF4G1 Antibody (NBP1-04965).
 

Research Areas for eIF4G1 Antibody (NBP1-04965)

Find related products by research area.

Blogs on eIF4G1

There are no specific blogs for eIF4G1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
01/11/2013
Application: WB
Species:

Bioinformatics

Gene Symbol EIF4G1
Entrez
Uniprot