eIF4G1 [p Ser1231] Antibody - BSA Free

Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke University.

Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - HT29 cells untreated or treated with Anisomycin.

Immunohistochemistry-Paraffin: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NBP1-04965 incubated with blocking peptide(right).

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

eIF4G1 [p Ser1231] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit eIF4G1 [p Ser1231] Antibody - BSA Free (NBP1-04965) is a polyclonal antibody validated for use in IHC and WB. Anti-eIF4G1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eIF4G1 [p Ser1231] around the phosphorylation site of serine 1231 (P-V-Sp-P-L).
• Modification: p Ser1231
• Specificity: Detects endogenous levels of eIF4G1 only when phosphorylated at serine 1231.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4G1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF4G1 [p Ser1231] Antibody - BSA Free

• DKFZp686A1451
• EIF4F
• eIF-4G 1
• eIF4G1
• EIF-4G1
• eIF-4-gamma 1
• EIF4-gamma
• EIF4GI
• EIF4Gp220
• eucaryotic translation initiation factor 4G
• eukaryotic translation initiation factor 4 gamma 1
• eukaryotic translation initiation factor 4 gamma, 1
• P220

Background

eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF4G1 Antibody (NBP1-04965)(3)

Publications using NBP1-04965

• Dobrikov MI, Shveygert M, Brown M, Gromeier M. Mitotic phosphorylation of eukaryotic initiation factor (eIF) 4G1 at Ser1232 by Cdk1:Cyclin B inhibits eIF4A helicase complex binding with RNA. Mol Cell Biol. 2013-11-18 [PMID: 24248602] (WB, Human)
• Dobrikov MI, Dobrikova EY, Gromeier M et al. Dynamic regulation of the translation initiation helicase complex by mitogenic signal transduction to eIF4G Mol Cell Biol 2012-12-21 [PMID: 23263986] (WB, Human)
• Dobrikov M, Dobrikova E, Shveygert M, Gromeier M. Phosphorylation of eIF4G1 by PKC{alpha} Regulates eIF4G1 Binding to Mnk1. Mol Cell Biol;31(14):2947-59. 2011-07-01 [PMID: 21576361]

Review for eIF4G1 Antibody (NBP1-04965) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer WB 01/11/2013

Summary

• Application: Western Blot

Bioinformatics

• Gene Symbol: EIF4G1
• Entrez:
  • 1981(Human)
• Uniprot:
  • Q04637()