eIF4A1 Antibody

Images

 
Western Blot: eIF4A1 Antibody [NBP2-24632] - Representative immunoblot of total lysate of MEFs with eIF4A (C-terminal epitope) and Tubulin antibodies in control and GS (glucose starvation). Image collected and cropped ...read more
Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Western Blot: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW cell lysate in the absence of immunizing peptide using NBP2-24632 ...read more
Characterization of the GS-induced AP of eIF4A. (F) Representative immunoblot of total lysate of MEFs with eIF4A (C-terminal epitope) and Tubulin antibodies in control and GS. Image collected and cropped by CiteAb from ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, PmSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

eIF4A1 Antibody Summary

Immunogen
A portion of amino acid 350-400 of human eIF4A1 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.25-1.0 ug/ml
Publications
Read Publications using
NBP2-24632 in the following applications:

  • WB
    2 publications

Reactivity Notes

Zebrafish (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for eIF4A1 Antibody

  • ATP-dependent RNA helicase eIF4A-1
  • DDX2Aeukaryotic initiation factor 4A-I
  • EC 3.6.1
  • EC 3.6.4.13
  • EIF4A
  • EIF-4A
  • eIF-4A-I
  • eIF4A-I
  • eukaryotic initiation factor 4AI
  • eukaryotic translation initiation factor 4A
  • eukaryotic translation initiation factor 4A, isoform 1
  • eukaryotic translation initiation factor 4A1

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for eIF4A1 Antibody (NBP2-24632)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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WB
(2)
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(1)
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional eIF4A1 Products

Array NBP2-24632

Research Areas for eIF4A1 Antibody (NBP2-24632)

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Blogs on eIF4A1

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Bioinformatics

Gene Symbol EIF4A1
Uniprot