eIF4GII Antibody

Images

 
Western Blot: eIF4GII Antibody [NBP2-16309] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: eIF4GII Antibody [NBP2-16309] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF4G3 protein stained by EIF4G3 antibody [N1], N-term diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded mouse brain stem. EIF4G3 antibody [N1], N-term dilution: 1:500.
Western Blot: eIF4GII Antibody [NBP2-16309] - Sample (30 ug of whole cell lysate) A: JC B: BCL-1 5% SDS PAGE gel, diluted at 1:5000.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Immunohistochemical analysis of paraffin-embedded AGS xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded mouse cervix. EIF4G3 antibody [N1], N-term dilution: 1:500.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP2-16309] - Paraffin-embedded rat hind brain. EIF4G3 antibody [N1], N-term dilution: 1:500.
Immunoprecipitation: eIF4GII Antibody [NBP2-16309] - Immunoprecipitation of EIF4G3 protein from 293T whole cell extracts using 5 ug of EIF4G3 antibody [N1], N-term. Western blot analysis was performed using EIF4G3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

eIF4GII Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EIF4G3. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4G3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
177 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF4GII Antibody

  • eIF-4G 3
  • eIF-4-gamma 3
  • eIF-4-gamma II
  • eIF4GIIeIF4G 3
  • eukaryotic translation initiation factor 4 gamma 3
  • eukaryotic translation initiation factor 4 gamma, 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF4G3
Uniprot