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HAF007). A specific band was detected for eIF4E at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF018). A specific band was detected for eIF4E at approximately 25 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).
![Immunocytochemistry eIF4E Antibody (299910) [Unconjugated]](http://images.novusbio.com/images/antibody/eIF4E_MAB3228_Immunocytochemistry_6605.jpg)
![Western Blot eIF4E Antibody (299910) [Unconjugated]](http://images.novusbio.com/images/antibody/eIF4E_MAB3228_Western_Blot_6076.jpg)
![Knockout Validated eIF4E Antibody (299910) [Unconjugated]](http://images.novusbio.com/images/antibody/eIF4E_MAB3228_Knockout_Validated_23536.jpg)
![Western Blot eIF4E Antibody (299910) [Unconjugated]](http://images.novusbio.com/fullsize/mab3228_human-mouse-rat-eif4e-mab-clone-299910-western-blot-12122025745315.jpg)
![Western Blot eIF4E Antibody (299910) [Unconjugated]](http://images.novusbio.com/fullsize/mab3228_human-mouse-rat-eif4e-mab-clone-299910-western-blot-121220257453113.jpg)
![Western Blot eIF4E Antibody (299910) [Unconjugated]](http://images.novusbio.com/fullsize/mab3228_human-mouse-rat-eif4e-mab-clone-299910-western-blot-12122025824121.jpg)
