EGLN1/PHD2 Antibody (366G/76/3)

Images

 
Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Western blot analysis in HeLa whole cell extracts using NBP1-30328.
Immunohistochemistry: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - IHC analysis of PHD2 in human renal cancer using DAB with hematoxylin counterstain.
Simple Western: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Simple Western lane view shows a specific band for EGLN1/PHD2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clone
366G/76/3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

EGLN1/PHD2 Antibody (366G/76/3) Summary

Immunogen
A peptide within residues 1-50 of human PHD2/HIF Prolyl Hydroxylase 2. [Swiss-Prot# Q9GZT9]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EGLN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This HIF Prolyl Hydroxylase 2 antibody is useful for Immunohistochemistry paraffin embedded sections, and Western Blot analysis where a band can be seen at approx. 46 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NBP1-30328 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Human. Immunogen sequence has 83% identity with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for EGLN1/PHD2 Antibody (366G/76/3)

  • C1orf12
  • C1orf12HPH2
  • EC 1.14.11
  • EC 1.14.11.-
  • ECYT3
  • egl nine homolog 1 (C. elegans)
  • egl nine homolog 1
  • egl nine-like protein 1
  • EGLN1
  • HIF prolyl hydroxylase 2
  • HIFPH2 EGL nine (C.elegans) homolog 1
  • HIFPH2
  • HIF-PH2
  • HIF-prolyl hydroxylase 2
  • HPH2
  • HPH-2
  • Hypoxia-inducible factor prolyl hydroxylase 2
  • PHD2
  • PHD2SM20
  • Prolyl hydroxylase domain-containing protein 2
  • SM20
  • SM-20
  • zinc finger MYND domain-containing protein 6
  • ZMYND6
  • ZMYND6DKFZp761F179

Background

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that mediate important physiological responses to hypoxia by modulating HIF1alpha levels. The HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). Under normoxic conditions, HIF Prolyl Hydroxylase 2 (HIF1-PH; also called PHD1 or Egln2) catalyzes the post-translational formation of 4-hydroxyproline through hydroxylation of a specific proline found in each of NODD/CODD domains of HIF1A and also hydroxylates HIF2A with preference for CODD site of HIF1A/HIF1B. After hydroxylation, HIFs undergo proteasomal degradation via VHL (von Hippel-Lindau) ubiquitination complex. However, under hypoxic conditions, the hydroxylation reaction is tempered which allows HIFs to escape degradation process followed by their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxia-inducible genes. HIF1-PH, through regulating HIF1 stability, is involved in various hypoxia-influenced processes such as angiogenesis in retinal/cardiac functionality as well as tumor angiogenesis, and defecticve EGLN1 have been linked to ECYT3 (familial erythrocytosis type 3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
NB100-139
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-P, IP
AF6394
Species: Hu
Applications: WB, IP
NB100-485
Species: Hu
Applications: WB, IP
NB100-122
Species: Hu, Mu, Rt, Fi, Ha, Pm, Sh
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NBP1-89843
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-428
Species: Hu, Mu, Rt
Applications: WB, EM, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB120-19347
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IF
NB100-124
Species: Hu, Mu, Rt, Bv, Ft, Pm, Sh
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP
NBP2-32964
Species: Hu
Applications: Flow, ICC/IF, IHC-Fr
NB100-417
Species: Hu, Mu, Rt, Ca, Pl
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-30328
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P

Publications for EGLN1/PHD2 Antibody (NBP1-30328)(4)

Reviews for EGLN1/PHD2 Antibody (NBP1-30328) (0)

There are no reviews for EGLN1/PHD2 Antibody (NBP1-30328). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for EGLN1/PHD2 Antibody (NBP1-30328): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EGLN1/PHD2 Antibody (NBP1-30328). (Showing 1 - 1 of 1 FAQs).

  1. Dear sirs, which anti-PHD3 antibody would you recommend for western blot of murine tissues? I have used the NB100-303 and I am able to detect the protein in cells, but not in heart samples. could you also recommend a protocol with Ab dilutions, solutions, transfer, etc?
    • In regards to your inquiry I am sorry to hear you have not had success with heart samples on NB100-303. We have another PHD3 antibody, NBP1-30328, that may be useful to you. It is guaranteed for mouse samples and has a sample size available if you wished to purchase and test first at a lower cost to you prior to committing to it.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-30328AF405
Alexa Fluor 488 Labeled NBP1-30328AF488
Alexa Fluor 647 Labeled NBP1-30328AF647
Alexa Fluor 700 Labeled NBP1-30328AF700
Biotin Labeled NBP1-30328B
DyLight 405 Labeled NBP1-30328V
DyLight 488 Labeled NBP1-30328G
DyLight 550 Labeled NBP1-30328R
DyLight 650 Labeled NBP1-30328C
FITC Labeled NBP1-30328F
HRP Labeled NBP1-30328H

Additional EGLN1/PHD2 Products

Bioinformatics Tool for EGLN1/PHD2 Antibody (NBP1-30328)

Discover related pathways, diseases and genes to EGLN1/PHD2 Antibody (NBP1-30328). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EGLN1/PHD2 Antibody (NBP1-30328)

Discover more about diseases related to EGLN1/PHD2 Antibody (NBP1-30328).
 

Pathways for EGLN1/PHD2 Antibody (NBP1-30328)

View related products by pathway.

PTMs for EGLN1/PHD2 Antibody (NBP1-30328)

Learn more about PTMs related to EGLN1/PHD2 Antibody (NBP1-30328).
 

Research Areas for EGLN1/PHD2 Antibody (NBP1-30328)

Find related products by research area.

Blogs on EGLN1/PHD2

There are no specific blogs for EGLN1/PHD2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EGLN1/PHD2 Antibody (366G/76/3) and receive a gift card or discount.

Bioinformatics

Gene Symbol EGLN1
Entrez
Uniprot