EGLN1/PHD2 Antibody (366G/76/3)

Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Aanalysis in HeLa whole cell extracts using NBP1-30328.

Immunohistochemistry: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Analysis of PHD2 in human renal cancer using DAB with hematoxylin counterstain.

Simple Western: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Llane view shows a specific band for EGLN1/PHD2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, IHC, IHC-P
• Clone: 366G/76/3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

EGLN1/PHD2 Antibody (366G/76/3) Summary

• Immunogen: A peptide within residues 1-50 of human PHD2/HIF Prolyl Hydroxylase 2. [Swiss-Prot# Q9GZT9]
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EGLN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2.0 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: This HIF Prolyl Hydroxylase 2 antibody is useful for Immunohistochemistry paraffin embedded sections, and Western Blot analysis where a band can be seen at approx. 46 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for EGLN1/PHD2 Antibody (366G/76/3)

• C1orf12
• C1orf12HPH2
• EC 1.14.11
• EC 1.14.11.-
• ECYT3
• egl nine homolog 1 (C. elegans)
• egl nine homolog 1
• egl nine-like protein 1
• EGLN1
• HIF prolyl hydroxylase 2
• HIFPH2 EGL nine (C.elegans) homolog 1
• HIFPH2
• HIF-PH2
• HIF-prolyl hydroxylase 2
• HPH2
• HPH-2
• Hypoxia-inducible factor prolyl hydroxylase 2
• PHD2
• PHD2SM20
• Prolyl hydroxylase domain-containing protein 2
• SM20
• SM-20
• zinc finger MYND domain-containing protein 6
• ZMYND6
• ZMYND6DKFZp761F179

Background

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that mediate important physiological responses to hypoxia by modulating HIF1alpha levels. The HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). Under normoxic conditions, HIF Prolyl Hydroxylase 2 (HIF1-PH; also called PHD1 or Egln2) catalyzes the post-translational formation of 4-hydroxyproline through hydroxylation of a specific proline found in each of NODD/CODD domains of HIF1A and also hydroxylates HIF2A with preference for CODD site of HIF1A/HIF1B. After hydroxylation, HIFs undergo proteasomal degradation via VHL (von Hippel-Lindau) ubiquitination complex. However, under hypoxic conditions, the hydroxylation reaction is tempered which allows HIFs to escape degradation process followed by their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxia-inducible genes. HIF1-PH, through regulating HIF1 stability, is involved in various hypoxia-influenced processes such as angiogenesis in retinal/cardiac functionality as well as tumor angiogenesis, and defecticve EGLN1 have been linked to ECYT3 (familial erythrocytosis type 3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EGLN1/PHD2 Antibody (NBP1-30328)(4)

Publications using NBP1-30328

• Jubb AM, Turley H, Moeller HC, Steers G, Han C, Li JL, Leek R, Tan EY, Singh B, Mortensen NJ, Noguera-Troise I, Pezzella F, Gatter KC, Thurston G, Fox SB, Harris AL. Expression of delta-like ligand 4 (Dll4) and markers of hypoxia in colon cancer. Br J Cancer;101(10):1749-57. 2009 Nov 17. [PMID: 19844231] (IHC-P, Human)
• Soilleux EJ et al. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Histopathology 47: 602-610. 2005 [PMID: 16324198] (IHC-P, Human)
• Stolze IP et al. Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (HIF) in regulating HIF transcriptional target genes. J Bio Chem 279:42719-42725. 2004 [PMID: 15302861] (WB, Human)
• Appelhoff RJ et al. Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor. J Bil Chem 279: 38458-38465. 2004 [PMID: 15247232] (WB, Human)

FAQs for EGLN1/PHD2 Antibody (NBP1-30328). (Showing 1 - 1 of 1 FAQs).

1. Dear sirs, which anti-PHD3 antibody would you recommend for western blot of murine tissues? I have used the NB100-303 and I am able to detect the protein in cells, but not in heart samples. could you also recommend a protocol with Ab dilutions, solutions, transfer, etc?
   • In regards to your inquiry I am sorry to hear you have not had success with heart samples on NB100-303. We have another PHD3 antibody, NBP1-30328, that may be useful to you. It is guaranteed for mouse samples and has a sample size available if you wished to purchase and test first at a lower cost to you prior to committing to it.

Bioinformatics

• Gene Symbol: EGLN1
• Entrez:
  • 54583(Human)
• Uniprot:
  • Q9GZT9(Human)