Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Western blot analysis in HeLa whole cell extracts using NBP1-30328.
Immunohistochemistry: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - IHC analysis of PHD2 in human renal cancer using DAB with hematoxylin counterstain.
Simple Western: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Simple Western lane view shows a specific band for EGLN1/PHD2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Protein G purified
Western Blot 0.5-2.0 ug/ml
Simple Western 1:50
This HIF Prolyl Hydroxylase 2 antibody is useful for Immunohistochemistry paraffin embedded sections, and Western Blot analysis where a band can be seen at ~46 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-30328 in the following applications:
Human. Immunogen sequence has 83% identity with mouse.
Alternate Names for EGLN1/PHD2 Antibody (366G/76/3)
egl nine homolog 1 (C. elegans)
egl nine homolog 1
egl nine-like protein 1
HIF prolyl hydroxylase 2
HIFPH2 EGL nine (C.elegans) homolog 1
HIF-prolyl hydroxylase 2
Hypoxia-inducible factor prolyl hydroxylase 2
Prolyl hydroxylase domain-containing protein 2
zinc finger MYND domain-containing protein 6
HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that mediate important physiological responses to hypoxia by modulating HIF1alpha levels. The HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). Under normoxic conditions, HIF Prolyl Hydroxylase 2 (HIF1-PH; also called PHD1 or Egln2) catalyzes the post-translational formation of 4-hydroxyproline through hydroxylation of a specific proline found in each of NODD/CODD domains of HIF1A and also hydroxylates HIF2A with preference for CODD site of HIF1A/HIF1B. After hydroxylation, HIFs undergo proteasomal degradation via VHL (von Hippel-Lindau) ubiquitination complex. However, under hypoxic conditions, the hydroxylation reaction is tempered which allows HIFs to escape degradation process followed by their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxia-inducible genes. HIF1-PH, through regulating HIF1 stability, is involved in various hypoxia-influenced processes such as angiogenesis in retinal/cardiac functionality as well as tumor angiogenesis, and defecticve EGLN1 have been linked to ECYT3 (familial erythrocytosis type 3).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for EGLN1/PHD2 Antibody (NBP1-30328). (Showing 1 - 1 of 1 FAQs).
Dear sirs, which anti-PHD3 antibody would you recommend for western blot of murine tissues? I have used the NB100-303 and I am able to detect the protein in cells, but not in heart samples. could you also recommend a protocol with Ab dilutions, solutions, transfer, etc?
In regards to your inquiry I am sorry to hear you have not had success with heart samples on NB100-303. We have another PHD3 antibody, NBP1-30328, that may be useful to you. It is guaranteed for mouse samples and has a sample size available if you wished to purchase and test first at a lower cost to you prior to committing to it.