FABP7/B-FABP Antibody [Unconjugated]

FABP7 was detected in immersion fixed paraffin-embedded sections of human melanoma using 5 µg/mL Goat Anti-Human FABP7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human cerebellum tissue and human hippocampus tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP7/B‑FABP at approximately 18 kDa (as indicated) using 50 µg/mL of Goat Anti-Human FABP7/B‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3166) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FABP7/B-FABP Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FABP7/B-FABP
  Met1-Ala132
  Accession # O15540
• Specificity: Detects human FABP7/B-FABP in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP6, rhFABP8, rhFABP9 and recombinant mouse FABP9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FABP7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP7/B-FABP Antibody [Unconjugated]

• BFABP
• B-FABP
• B-FABPDKFZp547J2313
• BLBP
• BLBPMRG
• Brain lipid-binding protein
• Brain-type fatty acid-binding protein
• FABP7
• FABPBbrain lipid binding protein
• fatty acid binding protein 7, brain
• Fatty acid-binding protein 7
• fatty acid-binding protein, brain
• Mammary-derived growth inhibitor related
• mammary-derived growth inhibitor-related

Background

FABP7, also known as (Brain) B-FABP, Brain lipid-binding protein (BLBP), and mammary-derived growth inhibitor-related (MRG), belongs to the family of intracellular lipid-binding proteins. It is involved in fatty acid uptake and intracellular transport and is important in brain development. Human and mouse FABP7 share 86% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FABP7/B-FABP Antibody (AF3166)(5)

Publications using AF3166

• Q Guo, I Kawahata, T Degawa, Y Ikeda-Mats, M Sun, F Han, K Fukunaga Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice Biomedicines, 2021;9(5):. 2021 [PMID: 34068550] (Western Blot, Mouse)
• K Zhang, F Yu, J Zhu, S Han, J Chen, X Wu, Y Chen, T Shen, J Liao, W Guo, X Yang, R Wang, Y Qian, J Yang, L Cheng, Y Zhao, CC Hui, J Li, G Peng, S He, N Jing, K Tang Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation Cell Rep, 2020;31(3):107521. 2020 [PMID: 32320667] (ICC, Human)
• T Owa, S Taya, S Miyashita, M Yamashita, T Adachi, K Yamada, M Yokoyama, S Aida, T Nishioka, YU Inoue, R Goitsuka, T Nakamura, T Inoue, K Kaibuchi, M Hoshino Meis1 coordinates cerebellar granule cell development by regulating Pax6 transcription, BMP signaling and Atoh1 degradation J. Neurosci., 2018;0(0):. 2018 [PMID: 29317485] (IHC-P, Mouse)
• N Takaoka, T Takayama, S Ozono Functional analysis of fatty acid binding protein 7 and its effect on fatty acid of renal cell carcinoma cell lines BMC Cancer, 2017;17(1):192. 2017 [PMID: 28292269] (Western Blot, Human)
• Boneva NB, Kaplamadzhiev DB, Sahara S, Kikuchi H, Pyko IV, Kikuchi M, Tonchev AB, Yamashima T Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys. Hippocampus, 2011;21(2):162-71. 2011 [PMID: 20014382] (Western Blot, Primate - Macaca fuscata (Japanese Macaque))

Reviews for FABP7/B-FABP Antibody (AF3166) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rat.

reviewed by: Verified Customer WB Rat 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21771320
• Species: Rat

Blocking

• Blocking Details: See PMID 21771320

Details

• Detection Notes: See PMID 21771320

Comments

• Comments: Published: See PMID 21771320Independent review from antibodyresource.com

reviewed by: Verified Customer ELISA Human 01/07/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 22646866
• Species: Human

Blocking

• Blocking Details: See PMID 22646866

Details

• Detection Notes: See PMID 22646866

Comments

• Comments: Published: See PMID 22646866Independent review from antibodyresource.com

Bioinformatics

• Gene Symbol: FABP7
• Uniprot:
  • O15540()