DNAJC2 Antibody - BSA Free

Western Blot: DNAJC2 Antibody [NBP2-12802] - Detection of human and mouse MPP11 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MPP11 antibody NBP2-12802 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MPP11 was also immunoprecipitated by rabbit anti-MPP11 antibody NBP2-12803, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DNAJC2 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit DNAJC2 Antibody - BSA Free (NBP2-12802) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-DNAJC2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 500 and 550 of human DnaJ (Hsp40) Homolog, Subfamily C, Member 2 using the numbering given in entry NP_055192.1 (GeneID 27000).
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNAJC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID 28402505)
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNAJC2 Antibody - BSA Free

• DnaJ (Hsp40) homolog, subfamily C, member 2
• dnaJ homolog subfamily C member 2
• MPHOSPH11M-phase phosphoprotein 11
• MPP11Zuotin-related factor 1
• ZRF1zuotin
• ZUO1
• zuotin related factor 1

Background

DNAJC2 is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNAJC2 Antibody (NBP2-12802)(3)

Publications using NBP2-12802

• De Magis A, Limmer M, Mudiyam V et al. UV-induced G4 DNA structures recruit ZRF1 which prevents UV-induced senescence Nature communications 2023-10-23 [PMID: 37872164] (KO)
• Chitale, SRichly, H, DICER- and MMSET-catalyzed H4K20me2 recruits the nucleotide excision repair factor XPA to DNA damage sites. J. Cell Biol. [PMID: 29233865] (IP, Human)
• Chitale S, Richly H. DICER and ZRF1 contribute to chromatin decondensation during nucleotide excision repair. Nucleic Acids Res. 2017-04-10 [PMID: 28402505] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: DNAJC2
• Uniprot:
  • Q99543(Human)