Histone H2a Antibody

Images

 
Western Blot: Histone H2a Antibody [NB100-56346] - WB for histone H2A in MCF-7 and HCT116 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Formalin-fixed, paraffin-embedded human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by ...read more
Western Blot: Histone H2a Antibody [NB100-56346] - analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Histone H2a Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acid residues 1-15 (MSGRGKQGGKARAKA) and 81-96 (PRHLQLAIRNDEELNK) of human Histone H2A.
Specificity
A 14 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H2AC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA 1:2000
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
ELISA: (see Dai et al, 2008 for details on Nucleosome ELISA)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56346 in the following applications:

Publications
Read Publications using NB100-56346.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Histone H2a Antibody

  • H2A histone family, member Q
  • H2A/q
  • H2A1B
  • H2AFQH2A
  • H2A-GL101
  • HIST1H2AB
  • histone 2, H2ac
  • histone cluster 2, H2ac
  • histone H2A type 2-C
  • Histone H2A
  • Histone H2A/q
  • Histone H2A-GL101
  • histone IIa
  • MGC74460

Background

Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, IHC-P
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Publications for Histone H2a Antibody (NB100-56346)(2)

Review for Histone H2a Antibody (NB100-56346) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56346:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Histone H2a NB100-56346
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reviewed by:
Anonymous
WB Human 07/31/2014
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Summary

ApplicationWestern Blot
Sample TestedMCF-7 and HCT116 cells whole cell extract
SpeciesHuman
CommentsThe antibody seems to detect the band at right size and one lighter band migrating slowly.

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:1000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 30 sec

Comments

CommentsThe antibody seems to detect the band at right size and one lighter band migrating slowly.

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FAQs for Histone H2a Antibody (NB100-56346) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Anonymous
07/31/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIST2H2AC
Entrez
Uniprot