OBFC2B Antibody (OTI1E11)

Western Blot: OBFC2B Antibody (1E11) [NBP2-01578] Analysis of extracts (35ug) from 9 different cell lines by using anti-OBFC2B monoclonal antibody.

Immunohistochemistry-Paraffin: OBFC2B Antibody (1E11) [NBP2-01578] - Staining of paraffin-embedded Human breast tissue using anti-OBFC2B mouse monoclonal antibody.

Flow Cytometry: OBFC2B Antibody (1E11) [NBP2-01578] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OBFC2B antibody, and then analyzed by flow cytometry.

Western Blot: OBFC2B Antibody (1E11) [NBP2-01578] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OBFC2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OBFC2B.

Immunohistochemistry-Paraffin: OBFC2B Antibody (1E11) [NBP2-01578] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OBFC2B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: OBFC2B Antibody (1E11) [NBP2-01578] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OBFC2B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: OBFC2B Antibody (1E11) [NBP2-01578] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-OBFC2B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: OBFC2B Antibody (1E11) [NBP2-01578] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OBFC2B mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.45 mg/ml

OBFC2B Antibody (OTI1E11) Summary

• Immunogen: Full length human recombinant protein of human OBFC2B(NP_076973) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NABP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 22.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.45 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OBFC2B Antibody (OTI1E11)

• LP3587
• MGC2731
• oligonucleotide/oligosaccharide-binding fold containing 2B
• Oligonucleotide/oligosaccharide-binding fold-containing protein 2B
• Sensor of single-strand DNA complex subunit B1
• Sensor of ssDNA subunit B1
• Single-stranded DNA-binding protein 1
• SOSS complex subunit B1
• SOSS-B1
• SSB1hSSB1

Background

Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage (Richard et al., 2008 [PubMed 18449195]).[supplied by OMIM]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NABP2
• Entrez:
  • 79035(Human)