DNA Ligase I Antibody

Images

 
Western Blot: DNA Ligase I Antibody [NB100-56635] - analysis of LIG1 in cell lysate from HeLa cells using this antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DNA Ligase I Antibody Summary

Immunogen
This antibody was developed against a synthetic peptide containing amino acids 51-68 of human LIG1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIG1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.1 mg/ml BSA.
Preservative
0.02% Sodium Azide
Purity
Protein G purified

Alternate Names for DNA Ligase I Antibody

  • DNA ligase 1
  • DNA ligase I
  • EC 6.5.1.1
  • ligase I, DNA, ATP-dependent
  • MGC117397
  • MGC130025
  • Polydeoxyribonucleotide synthase [ATP] 1
  • polydeoxyribonucleotide synthase ATP 1

Background

Five distinct DNA ligase activities, I-V, have been identified in mammalian cell extracts. One of these enzymes, LIG1 is central to DNA replication. During DNA replication, the joining of Okazaki fragments by the LIG1 gene product appears to be mediated by an interaction with proliferating cell nuclear antigen (PCNA). LIG1 participates in nucleotide excision and base excision repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-16182
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02280
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-41190
Species: Hu, Mu, Ch
Applications: WB, B/N, ICC/IF, IP, PLA
AF3495
Species: Hu
Applications: WB, IHC
NB100-119
Species: Hu, Mu
Applications: WB, ICC/IF, IP
AF3296
Species: Hu, Mu, Rt
Applications: WB
NB500-704
Species: Hu, Rt, ChHa
Applications: WB, ICC/IF, IHC, IHC-P
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP1-41190
Species: Hu, Mu, Ch
Applications: WB, B/N, ICC/IF, IP, PLA
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
NB100-74611
Species: Hu
Applications: WB, IHC-P, IP
NBP2-27335
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP2-38600
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-48053
Species: Hu
Applications: WB, IHC, IHC-P
NB100-477
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01020
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF3688
Species: Mu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-150
Species: Hu, Mu
Applications: WB, ICC/IF, IP
AF6349
Species: Hu, Mu, Rt
Applications: WB
NB100-56635
Species: Hu
Applications: WB
Supplier Logo

Publications for DNA Ligase I Antibody (NB100-56635) (0)

There are no publications for DNA Ligase I Antibody (NB100-56635).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DNA Ligase I Antibody (NB100-56635) (0)

There are no reviews for DNA Ligase I Antibody (NB100-56635). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DNA Ligase I Antibody (NB100-56635) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional DNA Ligase I Products

Bioinformatics Tool for DNA Ligase I Antibody (NB100-56635)

Discover related pathways, diseases and genes to DNA Ligase I Antibody (NB100-56635). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DNA Ligase I Antibody (NB100-56635)

Discover more about diseases related to DNA Ligase I Antibody (NB100-56635).
 

Pathways for DNA Ligase I Antibody (NB100-56635)

View related products by pathway.

PTMs for DNA Ligase I Antibody (NB100-56635)

Learn more about PTMs related to DNA Ligase I Antibody (NB100-56635).
 

Research Areas for DNA Ligase I Antibody (NB100-56635)

Find related products by research area.

Blogs on DNA Ligase I.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DNA Ligase I Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol LIG1
Uniprot