Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate-buffered saline, pH 7.2, containing no preservative
Protein G purified
Western Blot 1:100-1:2000
For WB: Use at an assay dependent dilution. Predicted molecular weight: 100 kDa.Blocking/Neutralizing was reported in scientific literature. Not tested in other applications. Optimal diutions/concentrations should be determined by the researcher.
Read Publications using NBP1-41190 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for DNA Ligase III Antibody (1F3)
DNA ligase 3
DNA ligase III
ligase II, DNA, ATP-dependent
ligase III, DNA, ATP-dependent
Polydeoxyribonucleotide synthase [ATP] 3
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa (beta-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.