LRIG1 Antibody [Unconjugated]

Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for LRIG1 at approximately 140 kDa and 70 kDa (as indicated, upper panel). For additional reference ERK1 was detected using Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF1575) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

LRIG1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

LRIG1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse LRIG1
  Ala37-Thr794
  Accession # P70193
• Specificity: Detects mouse LRIG1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) LRIG1 is observed and less than 5% cross-reactivity with rhLRIG3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Lrig1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRIG1 Antibody [Unconjugated]

• D6Bwg0781e
• Img
• LIG1
• LIG-1
• LRIG1

Background

LRIG-1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 130-145 kDa glycoprotein that belongs to the LRIG gene family.  It is widely expressed, and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons.  LRIG-1 is believed to negatively regulate the ErbB family of receptors.  In particular, and in a ligand-independent manner, LRIG-1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number.  Alternatively, LRIG-1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction.  Mature mouse LRIG-1 is a 1057 amino acid (aa) type I transmembrane protein (SwissProt #:P70193).  It contains a large 762 amino acid (aa) extracellular domain (ECD) (aa 35-795) plus a 274 aa cytoplasmic region.  The ECD contains 17 LRRs (aa’s 35-491) and three C2-type Ig-like domains (aa’s 497-781).  These two domain types are each sufficient for EGFR binding.  There one potential alternative splice form that a deletion of aa 875-923.  The LRIG-1 ECD undergoes proteolysis, generating 90-105 and 60-70 kDa soluble fragments.  Over aa 37-794, human LRIG-1 shares 97% and 90% aa sequence identity with rat and human LRIG-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRIG1 Antibody (AF3688)(24)

Publications using AF3688

• AE Lewis, A Kuwahara, J Franzosi, JO Bush Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 2022-03-15;38(11):110510. 2022-03-15 [PMID: 35294885] (IF, Mouse)
• J Bhuju, KM Olesen, CS Muenyi, TS Patel, RW Read, L Thompson, O Skalli, Q Zheng, EA Grice, CH Sutter, TR Sutter Cutaneous Effects of In Utero and Lactational Exposure of C57BL/6J Mice to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxics, 2021-08-20;9(8):. 2021-08-20 [PMID: 34437510] (IHC, Mouse)
• S Huang, P Kuri, Y Aubert, M Brewster, N Li, O Farrelly, G Rice, H Bae, S Prouty, T Dentchev, W Luo, BC Capell, P Rompolas Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization Cell Stem Cell, 2021-06-07;0(0):. 2021-06-07 [PMID: 34102139] (IHC, Mouse)
• D Jeong, D Lozano Cas, A Gengathara, K Edwards, A Saghatelya, DR Kaplan, FD Miller, SA Yuzwa LRIG1-Mediated Inhibition of EGF Receptor Signaling Regulates Neural Precursor Cell Proliferation in the Neocortex Cell Rep, 2020-10-13;33(2):108257. 2020-10-13 [PMID: 33053360] (ICC, IHC, Mouse)
• A Kuwahara, AE Lewis, C Coombes, FS Leung, M Percharde, JO Bush Delineating the early transcriptional specification of the mammalian trachea and esophagus Elife, 2020-06-09;9(0):. 2020-06-09 [PMID: 32515350] (IHC, Mouse)
• G Piedrafita, V Kostiou, A Wabik, B Colom, D Fernandez-, A Herms, K Murai, BA Hall, PH Jones A single-progenitor model as the unifying paradigm of epidermal and esophageal epithelial maintenance in mice Nat Commun, 2020-03-18;11(1):1429. 2020-03-18 [PMID: 32188860] (IHC, Mouse)
• Y Ge, Y Miao, S Gur-Cohen, N Gomez, H Yang, M Nikolova, L Polak, Y Hu, A Verma, O Elemento, JG Krueger, E Fuchs The aging skin microenvironment dictates stem cell behavior Proc. Natl. Acad. Sci. U.S.A., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094197] (IHC, Mouse)
• K Murai, G Skrupskely, G Piedrafita, M Hall, V Kostiou, SH Ong, T Nagy, A Cagan, D Goulding, AM Klein, BA Hall, PH Jones Epidermal Tissue Adapts to Restrain Progenitors Carrying Clonal p53 Mutations Cell Stem Cell, 2018-09-27;0(0):. 2018-09-27 [PMID: 30269904] (IHC, Mouse)
• K Singh, E Camera, L Krug, A Basu, RK Pandey, S Munir, M Wlaschek, S Kochanek, M Schorpp-Ki, M Picardo, P Angel, C Niemann, P Maity, K Scharffett JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin Nat Commun, 2018-08-24;9(1):3425. 2018-08-24 [PMID: 30143626] (IHC-P, Mouse)
• S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration Cell Stem Cell, 2017-12-14;22(1):35-49.e7. 2017-12-14 [PMID: 29249464] (IHC, Mouse)
• GL Chovatiya, RM Sarate, RR Sunkara, NP Gawas, V Kala, SK Waghmare Secretory phospholipase A2-IIA overexpressing mice exhibit cyclic alopecia mediated through aberrant hair shaft differentiation and impaired wound healing response Sci Rep, 2017-09-14;7(1):11619. 2017-09-14 [PMID: 28912581] (IHC, Mouse)
• JD Hoeck, B Biehs, AV Kurtova, NM Kljavin, F de Sousa E, B Alicke, H Koeppen, Z Modrusan, R Piskol, FJ de Sauvage Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss Nat. Cell Biol., 2017-05-29;19(6):666-676. 2017-05-29 [PMID: 28553937] (IHC, Mouse)
• Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs Stem Cell Lineage Infidelity Drives Wound Repair and Cancer Cell, 2017-04-20;0(0):. 2017-04-20 [PMID: 28434617] (IHC, Mouse)
• Petersson , Monika, Reuter , Karen, Brylka , Heike, Kraus , Andreas, Schettina , Peter, Niemann , Catherin Interfering with stem cell-specific gatekeeper functions controls tumour initiation and malignant progression of skin tumours. Nat Commun, 2015-01-22;6(0):5874. 2015-01-22 [PMID: 25608467] (IHC, Mouse)
• Zhao , Huashan, Qiao , Jingqiao, Zhang , Shoubing, Zhang , Huishan, Lei , Xiaohua, Wang , Xinyue, Deng , Zhili, Ning , Lina, Cao , Yujing, Guo , Yong, Liu , Shuang, Duan , Enkui GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing. Sci Rep, 2015-01-21;5(0):7913. 2015-01-21 [PMID: 25604641] (IHC-Fr, Mouse)
• Dahlhoff M, Frances D, Kloepper J, Paus R, Schafer M, Niemann C, Schneider M Overexpression of epigen during embryonic development induces reversible, epidermal growth factor receptor-dependent sebaceous gland hyperplasia. Mol Cell Biol, 2014-06-02;34(16):3086-95. 2014-06-02 [PMID: 24891618] (IHC-Fr, Mouse)
• Rognoni E, Widmaier M, Jakobson M, Ruppert R, Ussar S, Katsougkri D, Bottcher R, Lai-Cheong J, Rifkin D, McGrath J, Fassler R Kindlin-1 controls Wnt and TGF-beta availability to regulate cutaneous stem cell proliferation. Nat Med, 2014-03-30;20(4):350-9. 2014-03-30 [PMID: 24681597] (IHC, IHC-P, Mouse)
• Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013-12-12;504(7479):277-81. 2013-12-12 [PMID: 24336287] (IHC-Fr, Mouse)
• Maddox , Jessica, Shakya , Arvind, South , Samuel, Shelton , Dawne, Andersen , Jared N, Chidester , Stephani, Kang , Jinsuk, Gligorich , Keith M, Jones , David A, Spangrude , Gerald J, Welm , Bryan E, Tantin , Dean Transcription factor Oct1 is a somatic and cancer stem cell determinant. PLoS Genet, 2012-11-08;8(11):e1003048. 2012-11-08 [PMID: 23144633] (IHC-Fr, Mouse)
• Wong , Vivian W, Stange , Daniel E, Page , Mahalia, Buczacki , Simon, Wabik , Agnieszk, Itami , Satoshi, van de Wetering , Marc, Poulsom , Richard, Wright , Nicholas, Trotter , Matthew, Watt , Fiona M, Winton , Doug J, Clevers , Hans, Jensen , Kim B Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling. Nat Cell Biol, 2012-03-04;14(4):401-8. 2012-03-04 [PMID: 22388892] (IHC-P, Mouse)
• Liang X, Bhattacharya S, Bajaj G Delayed cutaneous wound healing and aberrant expression of hair follicle stem cell markers in mice selectively lacking Ctip2 in epidermis. PLoS ONE, 2012-02-21;7(2):e29999. 2012-02-21 [PMID: 22383956] (IHC, Mouse)
• Petersson M, Brylka H, Kraus A TCF/Lef1 activity controls establishment of diverse stem and progenitor cell compartments in mouse epidermis. EMBO J., 2011-06-21;30(15):3004-18. 2011-06-21 [PMID: 21694721] (Flow Cytometry, Mouse)
• Jensen KB, Driskell RR, Watt FM Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis. Nat Protoc, 2010-04-22;5(5):898-911. 2010-04-22 [PMID: 20431535] (Flow Cytometry, Mouse)
• Baker CM, Verstuyf A, Jensen KB Differential sensitivity of epidermal cell subpopulations to beta-catenin-induced ectopic hair follicle formation. Dev. Biol., 2010-04-14;343(1):40-50. 2010-04-14 [PMID: 20398648] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: Lrig1
• Uniprot:
  • P70193()