Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse ...read more
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more
LRIG1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Detects mouse LRIG1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) LRIG1 is observed and less than 5% cross-reactivity with rhLRIG3 is observed.
Immunogen affinity purified
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for LRIG1 Antibody [Unconjugated]
LRIG-1 (leucine-rich repeats and Ig-like domains-1; also
LIG-1) is an approximately 130-145 kDa glycoprotein that belongs to the LRIG
gene family. It is widely expressed, and
appears on the surface of prostatic epithelium, endothelial cells, vascular and
visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and
neurons. LRIG-1 is believed to negatively
regulate the ErbB family of receptors.
In particular, and in a ligand-independent manner, LRIG-1 complexes with
all four ErbBs, promoting their ubiquitination and decreasing their
number. Alternatively, LRIG-1 is suggested
to bind to the ErbBs, preventing their dimerization and signal
transduction. Mature mouse LRIG-1 is a
1057 amino acid (aa) type I transmembrane protein (SwissProt #:P70193). It contains a large 762 amino acid (aa)
extracellular domain (ECD) (aa 35-795) plus a 274 aa cytoplasmic region. The ECD contains 17 LRRs (aa’s 35-491) and
three C2-type Ig-like domains (aa’s 497-781).
These two domain types are each sufficient for EGFR binding. There one potential alternative splice form
that a deletion of aa 875-923. The LRIG-1
ECD undergoes proteolysis, generating 90-105 and 60-70 kDa soluble
fragments. Over aa 37-794, human LRIG-1 shares
97% and 90% aa sequence identity with rat and human LRIG-1, respectively.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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