LRIG1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of bEnd.3 mouse endothelioma cell line untreated (-), treated (+) or mock treated (m) with 30 pmol mouse LRIG1 or mouse ERK1 siRNA. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse ...read more
LRIG1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse LRIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3688) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse LRIG1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

LRIG1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LRIG1
Ala37-Thr794
Accession # P70193
Specificity
Detects mouse LRIG1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) LRIG1 is observed and less than 5% cross-reactivity with rhLRIG3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Lrig1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF3688 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRIG1 Antibody [Unconjugated]

  • D6Bwg0781e
  • Img
  • LIG1
  • LIG-1
  • LRIG1

Background

LRIG-1 (leucine-rich repeats and Ig-like domains-1; also LIG-1) is an approximately 130-145 kDa glycoprotein that belongs to the LRIG gene family.  It is widely expressed, and appears on the surface of prostatic epithelium, endothelial cells, vascular and visceral smooth muscle, mammary epithelium, cardiac muscle, keratinocytes and neurons.  LRIG-1 is believed to negatively regulate the ErbB family of receptors.  In particular, and in a ligand-independent manner, LRIG-1 complexes with all four ErbBs, promoting their ubiquitination and decreasing their number.  Alternatively, LRIG-1 is suggested to bind to the ErbBs, preventing their dimerization and signal transduction.  Mature mouse LRIG-1 is a 1057 amino acid (aa) type I transmembrane protein (SwissProt #:P70193).  It contains a large 762 amino acid (aa) extracellular domain (ECD) (aa 35-795) plus a 274 aa cytoplasmic region.  The ECD contains 17 LRRs (aa’s 35-491) and three C2-type Ig-like domains (aa’s 497-781).  These two domain types are each sufficient for EGFR binding.  There one potential alternative splice form that a deletion of aa 875-923.  The LRIG-1 ECD undergoes proteolysis, generating 90-105 and 60-70 kDa soluble fragments.  Over aa 37-794, human LRIG-1 shares 97% and 90% aa sequence identity with rat and human LRIG-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LRIG1 Antibody (AF3688)(15)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Flow, IHC, IHC Frozen, IHC Frozen, IHC Paraffin-embedded, IHC-P.


Filter By Application
Flow
(2)
IHC
(5)
IHC Frozen
(1)
IHC Frozen
(4)
IHC Paraffin-embedded
(2)
IHC-P
(1)
All Applications
Filter By Species
Mouse
(15)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF3688 Applications Species
K Singh, E Camera, L Krug, A Basu, RK Pandey, S Munir, M Wlaschek, S Kochanek, M Schorpp-Ki, M Picardo, P Angel, C Niemann, P Maity, K Scharffett JunB defines functional and structural integrity of the epidermo-pilosebaceous unit in the skin Nat Commun, 2018;9(1):3425. 2018 [PMID: 30143626] (IHC-P,, Mouse) IHC-P Mouse
S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration Cell Stem Cell, 2018;22(1):35-49.e7. 2018 [PMID: 29249464] (IHC,, Mouse) IHC Mouse
GL Chovatiya, RM Sarate, RR Sunkara, NP Gawas, V Kala, SK Waghmare Secretory phospholipase A2-IIA overexpressing mice exhibit cyclic alopecia mediated through aberrant hair shaft differentiation and impaired wound healing response Sci Rep, 2017;7(1):11619. 2017 [PMID: 28912581] (IHC,, Mouse) IHC Mouse
JD Hoeck, B Biehs, AV Kurtova, NM Kljavin, F de Sousa E, B Alicke, H Koeppen, Z Modrusan, R Piskol, FJ de Sauvage Stem cell plasticity enables hair regeneration following Lgr5(+) cell loss Nat. Cell Biol., 2017;19(6):666-676. 2017 [PMID: 28553937] (IHC,, Mouse) IHC Mouse
Y Ge, NC Gomez, RC Adam, M Nikolova, H Yang, A Verma, CP Lu, L Polak, S Yuan, O Elemento, E Fuchs Stem Cell Lineage Infidelity Drives Wound Repair and Cancer Cell, 2017;0(0):. 2017 [PMID: 28434617] (IHC,, Mouse) IHC Mouse
Zhao, Huashan, Qiao, Jingqiao, Zhang, Shoubing, Zhang, Huishan, Lei, Xiaohua, Wang, Xinyue, Deng, Zhili, Ning, Lina, Cao, Yujing, Guo, Yong, Liu, Shuang, Duan, Enkui GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing. Sci Rep, 2015;5(0):7913. 2015 [PMID: 25604641] (IHC Frozen,, Mouse) IHC Frozen Mouse
Dahlhoff M, Frances D, Kloepper J, Paus R, Schafer M, Niemann C, Schneider M Overexpression of epigen during embryonic development induces reversible, epidermal growth factor receptor-dependent sebaceous gland hyperplasia. Mol Cell Biol, 2014;34(16):3086-95. 2014 [PMID: 24891618] (IHC Frozen,, Mouse) IHC Frozen Mouse
Rognoni E, Widmaier M, Jakobson M, Ruppert R, Ussar S, Katsougkri D, Bottcher R, Lai-Cheong J, Rifkin D, McGrath J, Fassler R Kindlin-1 controls Wnt and TGF-beta availability to regulate cutaneous stem cell proliferation. Nat Med, 2014;20(4):350-9. 2014 [PMID: 24681597] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013;504(7479):277-81. 2013 [PMID: 24336287] (IHC Frozen,, Mouse) IHC Frozen Mouse
Maddox, Jessica, Shakya, Arvind, South, Samuel, Shelton, Dawne, Andersen, Jared N, Chidester, Stephani, Kang, Jinsuk, Gligorich, Keith M, Jones, David A, Spangrude, Gerald J, Welm, Bryan E, Tantin, Dean Transcription factor Oct1 is a somatic and cancer stem cell determinant. PLoS Genet, 2012;8(11):e1003048. 2012 [PMID: 23144633] (IHC Frozen,, Mouse) IHC Frozen Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol Lrig1
Uniprot