XRCC4 Antibody

Images

 
Western Blot: XRCC4 Antibody [NBP1-89463] - Analysis in control (vector only transfected HEK293T lysate) and XRCC4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: XRCC4 Antibody [NBP1-89463] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Analysis in human testis and liver tissues using NBP1-89463 antibody. Corresponding XRCC4 RNA-seq data are presented for the same tissues.
Genetic Strategies: Western Blot: XRCC4 Antibody [NBP1-89463] - Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is ...read more
Genetic Strategies: Western Blot: XRCC4 Antibody [NBP1-89463] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC4 antibody. Remaining relative ...read more
Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

XRCC4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEK
Specificity
Specificity of human XRCC4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XRCC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04 - 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control
XRCC4 Knockout HeLa Cell Lysate
Control Peptide
XRCC4 Protein (NBP1-89463PEP)
Publications
Read Publications using
NBP1-89463 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for XRCC4 Antibody

  • complementing defective, repair in Chinese hamster
  • X-ray repair complementing defective repair in Chinese hamster cells 4
  • X-ray repair cross-complementing protein 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-89463
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD

Publications for XRCC4 Antibody (NBP1-89463)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XRCC4 Antibody (NBP1-89463) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for XRCC4 Antibody (NBP1-89463)

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Blogs on XRCC4.

Ku70: The DNA's Mr. Fix-it
Ku70, known by several synonyms including X-ray repair cross-complementing, 5'-deoxyribose-5-phosphate lyase Ku70 protein 6, 70 kDa subunit of Ku antigen, XRCC6, and G22P1, is a 70 kDa protein that was shown to be involved in multiple cellular pathway...  Read full blog post.

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Bioinformatics

Gene Symbol XRCC4