XRCC4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: XRCC4 Antibody [NBP1-89463] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Analysis in human testis and liver tissues using NBP1-89463 antibody. Corresponding XRCC4 RNA-seq data are presented for the same tissues.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human kidney shows moderate nuclear positivity in cells in tubules.

Staining of human small intestine shows strong nuclear positivity in glandular cells.

Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

XRCC4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit XRCC4 Antibody - BSA Free (NBP1-89463) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-XRCC4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XRCC4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  XRCC4 Protein (NBP1-89463PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for XRCC4 Antibody - BSA Free

• complementing defective, repair in Chinese hamster
• X-ray repair complementing defective repair in Chinese hamster cells 4
• X-ray repair cross-complementing protein 4

Background

XRCC4 is encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XRCC4 Antibody (NBP1-89463)(2)

Publications using NBP1-89463

• Reid DA, Keegan S, Leo-Macias A et al. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proc Natl Acad Sci U S A 2015-05-19 [PMID: 25941401] (WB, ICC/IF, Human)
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: XRCC4