Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

Immunohistochemistry;Dihydrolipoamide Dehydrogenase/DLD Antibody[NBP3-30341]Detection of human DLD by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DLD used at a dilution of 1:1,000 . Detection: DAB

Immunohistochemistry;Dihydrolipoamide Dehydrogenase/DLD Antibody[NBP3-30391]Detection of human DLD by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DLD used at a dilution of 1:5,000 . Detection: DAB

Immunoprecipitation:Dihydrolipoamide Dehydrogenase/DLD AntibodyNBP3-30391] -Detection of human DLD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DLD antibody NBP3-30391 (lot NBP3-30391-1) used for IP at 6 µg per reaction. DLD was also immunoprecipitated by rabbit anti-DLD antibody. For blotting immunoprecipitated DLD, NBP3-30391 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free (NBP3-30391) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 459 and 509
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLD
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Dihydrolipoamide Dehydrogenase/DLD Antibody - BSA Free

• 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
• branched chain keto acid dehydrogenase complex
• Diaphorase
• dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex
• Dihydrolipoamide Dehydrogenase
• dihydrolipoamide dehydrogenasemitochondrial
• DLD
• DLDD
• DLDH
• E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex
• E3
• EC 1.8.1
• EC 1.8.1.4
• GCSL
• Glycine cleavage system L protein
• glycine cleavage system protein L
• LAD
• Lipoamide Dehydrogenase
• lipoamide reductase
• lipoyl dehydrogenase
• PHE3

Background

Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLD