Dihydrolipoamide Dehydrogenase/DLD Antibodies
We offer Dihydrolipoamide Dehydrogenase/DLD Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Dihydrolipoamide Dehydrogenase/DLD Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Dihydrolipoamide Dehydrogenase/DLD Antibodies can be used in a variety of model species: Human, Mouse, Porcine. Use the list below to choose the Dihydrolipoamide Dehydrogenase/DLD Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Dihydrolipoamide Dehydrogenase/DLD polyclonal antibodies and browse our Dihydrolipoamide Dehydrogenase/DLD monoclonal antibody catalog.
Alternate Names for Dihydrolipoamide Dehydrogenase/DLD Antibodies
anti-Dihydrolipoamide Dehydrogenase/DLD antibody, anti-DLD antibody, anti-2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex) antibody, anti-branched chain keto acid dehydrogenase complex antibody, anti-dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex antibody, anti-dihydrolipoamide dehydrogenasemitochondrial antibody, anti-DLDH antibody, anti-E3 antibody, anti-E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex antibody, anti-EC 1.8.1 antibody, anti-EC 1.8.1.4 antibody, anti-GCSL antibody, anti-Glycine cleavage system L protein antibody, anti-glycine cleavage system protein L antibody, anti-lipoamide dehydrogenase antibody, anti-lipoamide reductase antibody, anti-lipoyl dehydrogenase antibody
18 Results for "dihydrolipoamide-dehydrogenase-dld" in Primary Antibodies
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Dihydrolipoamide Dehydrogenase/DLD was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Dihydrolipoamide Dehydrogenase/DLD Monoclonal Antibody (Catalog # MAB8646) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Human gastric cancer, using DLD antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunohistochemistry-Paraffin-NBP1-31302-img0008.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0010.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample(30 ug of whole cell lysate) A:293T 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0011.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0012.jpg)
![Western Blot: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoamide Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lipoamide Dehydrogenase.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Western-Blot-NBP2-01109-img0002.jpg "Western Blot: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoamide Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lipoamide Dehydrogenase.")
![Immunocytochemistry/Immunofluorescence: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Lipoamide Dehydrogenase.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunocytochemistry-Immunofluorescence-NBP2-01109-img0007.jpg)
![Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human pancreas tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunohistochemistry-Paraffin-NBP2-01109-img0006.jpg)
![Flow Cytometry: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Lipoamide Dehydrogenase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Flow-Cytometry-NBP2-01109-img0001.jpg)
![Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunohistochemistry-Paraffin-NBP2-01109-img0003.jpg)
![Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human endometrium tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunohistochemistry-Paraffin-NBP2-01109-img0004.jpg)
![Immunohistochemistry-Paraffin: Lipoamide Dehydrogenase Antibody (6D5) [NBP2-01109] - Staining of paraffin-embedded Human lymph node tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-6D5-Immunohistochemistry-Paraffin-NBP2-01109-img0005.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Western blot analysis of extracts of various cell lines, using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb (NBP3-16595) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-3J2O9-Western-Blot-NBP3-16595-img0006.jpg "Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Western blot analysis of extracts of various cell lines, using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb (NBP3-16595) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Immunofluorescence analysis of U-2 OS cells using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb (NBP3-16595) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-3J2O9-Immunocytochemistry-Immunofluorescence-NBP3-16595-img0005.jpg)
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Immunohistochemistry analysis of Dihydrolipoamide Dehydrogenase/DLD in paraffin-embedded human colon carcinoma tissue using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16595_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-3j2o9-72202512435319.jpg)
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Immunohistochemistry analysis of Dihydrolipoamide Dehydrogenase/DLD in paraffin-embedded rat spleen tissue using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16595_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-3j2o9-7220251245367.jpg)
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Immunohistochemistry analysis of Dihydrolipoamide Dehydrogenase/DLD in paraffin-embedded human liver tissue using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16595_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-3j2o9-7220251245348.jpg)
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Immunohistochemistry analysis of Dihydrolipoamide Dehydrogenase/DLD in paraffin-embedded human kidney tissue using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16595_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-3j2o9-72202512432099.jpg)
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Immunohistochemistry analysis of Dihydrolipoamide Dehydrogenase/DLD in paraffin-embedded human esophagus tissue using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16595_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-3j2o9-72202512443358.jpg)
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Immunohistochemistry analysis of Dihydrolipoamide Dehydrogenase/DLD in paraffin-embedded mouse kidney tissue using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16595_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-3j2o9-7220251245399.jpg)
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (3J2O9) [NBP3-16595] - Immunohistochemistry analysis of Dihydrolipoamide Dehydrogenase/DLD in paraffin-embedded human tonsil tissue using Dihydrolipoamide Dehydrogenase/DLD Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16595_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-3j2o9-72202512432031.jpg)
![Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] - Analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-19361-img0002.jpg "Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] - Analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.")
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Lipoamide-Dehydrogenase-Antibody-Western-Blot-NBP2-19361-img0001.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-19361] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP2-19361-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody [N1N3] (NBP2-19361) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19361_rabbit-polyclonal-dihydrolipoamide-dehydrogenase-dld-antibody-western-blot-2611202518381515.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP2-13926-img0005.jpg "Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10<br/>Lane 2: Human Cerebral Cortex tissue")
![Immunocytochemistry/Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13926-img0006.jpg)
![Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunohistochemistry-Paraffin-NBP2-13926-img0008.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)<br/>Lane 5: Human liver tissue<br/>Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP2-13926-img0004.jpg)
![Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Staining of human epididymis shows low expression as expected.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunohistochemistry-Paraffin-NBP2-13926-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP2-13926] - Staining in human kidney and epididymis tissues using anti-DLD antibody. Corresponding DLD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunohistochemistry-Paraffin-NBP2-13926-img0009.jpg)
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # ![Immunohistochemistry;Dihydrolipoamide Dehydrogenase/DLD Antibody[NBP3-30341]Detection of human DLD by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DLD used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30391_rabbit-dihydrolipoamide-dehydrogenase-dld-pab-78202410153550.jpg "Immunohistochemistry;Dihydrolipoamide Dehydrogenase/DLD Antibody[NBP3-30341]Detection of human DLD by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DLD used at a dilution of 1:1,000 . Detection: DAB")
![Immunohistochemistry;Dihydrolipoamide Dehydrogenase/DLD Antibody[NBP3-30391]Detection of human DLD by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DLD used at a dilution of 1:5,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30391_rabbit-dihydrolipoamide-dehydrogenase-dld-pab-78202410372614.jpg)
![Immunoprecipitation:Dihydrolipoamide Dehydrogenase/DLD AntibodyNBP3-30391] -Detection of human DLD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DLD antibody NBP3-30391 (lot NBP3-30391-1) used for IP at 6 µg per reaction. DLD was also immunoprecipitated by rabbit anti-DLD antibody. For blotting immunoprecipitated DLD, NBP3-30391 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-30391_rabbit-dihydrolipoamide-dehydrogenase-dld-pab-3012202418203110.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (3C1) [H00001738-M02] - DLD monoclonal antibody (M02), clone 3C1 Analysis of DLD expression in Hela.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-3C1-Western-Blot-H00001738-M02-img0010.jpg "Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (3C1) [H00001738-M02] - DLD monoclonal antibody (M02), clone 3C1 Analysis of DLD expression in Hela.")
![Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody (3C1) [H00001738-M02] - Analysis of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-3C1-Immunohistochemistry-Paraffin-H00001738-M02-img0008.jpg)
![ELISA: Dihydrolipoamide Dehydrogenase/DLD Antibody (3C1) [H00001738-M02] - Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-3C1-ELISA-H00001738-M02-img0009.jpg)
![Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32539) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32539_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-psh0-83-29820241492614.jpg "Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32539) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunocytochemistry analysis of HeLa cells labeling Dihydrolipoamide Dehydrogenase/DLD with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/200 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32539_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-psh0-83-29202410234295.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (SR2009) [NBP3-22284] - Western blot analysis using NBP3-22284 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-22284_rabbit-dihydrolipoamide-dehydrogenase-dld-mab-sr2009-57202317215173.jpg "Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (SR2009) [NBP3-22284] - Western blot analysis using NBP3-22284 in 293T cell lysate.")
![Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody (1G11) [H00001738-M04] - Analysis of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-1G11-Immunohistochemistry-Paraffin-H00001738-M04-img0005.jpg "Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody (1G11) [H00001738-M04] - Analysis of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml")
![Sandwich ELISA: Dihydrolipoamide Dehydrogenase/DLD Antibody (1G11) [H00001738-M04] - Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-1G11-Sandwich-ELISA-H00001738-M04-img0006.jpg)
![Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody (2D4) [H00001738-M05] - Analysis of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-2D4-Immunohistochemistry-Paraffin-H00001738-M05-img0006.jpg "Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody (2D4) [H00001738-M05] - Analysis of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml")
![ELISA: Dihydrolipoamide Dehydrogenase/DLD Antibody (2D4) [H00001738-M05] - Detection limit for recombinant GST tagged DLD is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-2D4-ELISA-H00001738-M05-img0003.jpg)
