UBE4A Antibody (1G8) - Azide and BSA Free

Western Blot: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of UBE4A expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of monoclonal antibody to UBE4A on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of UBE4A expression in PC-12.

Western Blot: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of UBE4A expression in K-562.

ELISA: UBE4A Antibody (1G8) [H00009354-M08] - Detection limit for recombinant GST tagged UBE4A is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 1G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UBE4A Antibody (1G8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse UBE4A Antibody (1G8) - Azide and BSA Free (H00009354-M08) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: UBE4A (NP_004779, 974 a.a. ~ 1073 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE
• Specificity: UBE4A (1G8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBE4A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UBE4A Antibody (1G8) - Azide and BSA Free

• E4MGC133315
• KIAA0126ubiquitination factor E4A (homologous to yeast UFD2)
• ubiquitination factor E4A (UFD2 homolog, yeast)
• UBOX2
• UFD2ubiquitin conjugation factor E4 A

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE4A
• Entrez:
  • 9354(Human)
• OMIM:
  • 603753(Human)
• Uniprot:
  • NP_004779(Human)