Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoamide Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant

Immunocytochemistry/Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Lipoamide Dehydrogenase.

Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - Staining of paraffin-embedded Human lymph node tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.

Flow Cytometry: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Lipoamide Dehydrogenase antibody, and then analyzed by flow cytometry.

Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.

Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - Staining of paraffin-embedded Human endometrium tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.

Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - Staining of paraffin-embedded Human pancreas tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI6D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free (NBP2-01109) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human DLD(NP_000099) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 50.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free

• 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
• branched chain keto acid dehydrogenase complex
• Diaphorase
• dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex
• Dihydrolipoamide Dehydrogenase
• dihydrolipoamide dehydrogenasemitochondrial
• DLD
• DLDD
• DLDH
• E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex
• E3
• EC 1.8.1
• EC 1.8.1.4
• GCSL
• Glycine cleavage system L protein
• glycine cleavage system protein L
• LAD
• Lipoamide Dehydrogenase
• lipoamide reductase
• lipoyl dehydrogenase
• PHE3

Background

Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLD